Changes in Caprine Milk Oligosaccharides at Different Lactation Stages Analyzed by High Performance Liquid Chromatography Coupled to Mass Spectrometry.
Changes in Caprine Milk Oligosaccharides at Different Lactation Stages Analyzed by High Performance Liquid Chromatography Coupled to Mass Spectrometry.
复制标题
DOI：
10.1021/acs.jafc.6b05104
复制
发表时间：
2017-05-03
影响因子：
6.1
通讯作者：
Sanz ML
中科院分区：
文献类型：
--
作者：
Martín-Ortiz A;Barile D;Salcedo J;Moreno FJ;Clemente A;Ruiz-Matute AI;Sanz ML
采用亲水作用液相色谱-质谱联用技术（HILIC-Q MS）和纳米流液相色谱-四极杆-飞行时间质谱联用技术（nano-LC-Chip-QTOF MS）研究了山羊乳中低聚糖（CMO）在不同泌乳阶段的含量变化。通过HILIC-Q MS在不同泌乳阶段定量了8种主要寡糖（OS），而使用nano-LC-Chip-QToF MS可以通过监测中性非岩藻糖基化和岩藻糖基化物质以及不仅含有N-乙酰神经氨酸或N-羟乙酰神经氨酸残基而且含有两种唾液酸组合的酸性物质将研究扩展到49种不同的OS。总的来说，最丰富的OS随着泌乳时间的推移而减少，而观察到不同的趋势。6′-唾液酸-乳糖是最丰富的酸性OS，而半乳糖基-乳糖异构体被确定为最丰富的中性OS。
Changes of the abundance of caprine milk oligosaccharides (CMO) at different lactation stages have been evaluated by hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC–Q MS) and nanoflow liquid chromatography–quadrupole-time-of-flight mass spectrometry (nano-LC-Chip–QTOF MS). Eight major oligosaccharides (OS) were quantified at different lactation stages by HILIC–Q MS, while the use of nano-LC-Chip–QToF MS allowed expanding the study to forty-nine different OS by monitoring neutral non- and fucosylated species, as well as acidic species containing not only N-acetyl-neuraminic acid or N-glycolyl-neuraminic acid residues but also the combination of both sialic acids. Overall, the most abundant OS decreased with lactation time, whereas different trends were observed for minor OS. 6′-Sialyl-lactose was the most abundant acidic OS while galactosyl-lactose isomers were identified as the most abundant neutral OS. This is the first time that a comprehensive study regarding the changes of the abundance of CMO, both neutral and acidic, at different lactation stages is carried out.
登录
查看更多内容
影响因子：
6.1
作者：
Liu, Zhiqian;Moate, Peter;Rochfort, Simone
通讯作者：
Rochfort, Simone
影响因子：
3.1
作者：
Martinez-Ferez, A;Rudloff, S;Kunz, C
通讯作者：
Kunz, C
影响因子：
6.1
作者：
Locascio, Riccardo G.;Ninonuevo, Milady R.;German, J. Bruce
通讯作者：
German, J. Bruce
影响因子：
4.1
作者：
Hernandez-Hernandez, O.;Calvillo, I.;Sanz, M. L.
通讯作者：
Sanz, M. L.
影响因子：
6.1
作者：
Fong, Bertram;Ma, Kevin;McJarrow, Paul
通讯作者：
McJarrow, Paul