Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds.
Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds.
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羽衣甘蓝种子形成过程中芥子油苷代谢基因的比较转录组分析
DOI：
10.1186/s12870-021-03168-2
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发表时间：
2021-08-21
影响因子：
5.3
通讯作者：
Guo R
中科院分区：
文献类型：
--
作者：
Zhao Y;Chen Z;Chen J;Chen B;Tang W;Chen X;Lai Z;Guo R
背景为了解硫代葡萄糖苷（glucosinolates，GS）在特定器官中的积累机制，本研究采用生理变化分析和转录组测序相结合的方法。以芥蓝种子为材料，根据种子胚的发育过程，将种子和角果壁划分为不同的发育阶段，进行GS分析和转录组测序。在胚从鱼雷胚向子叶胚早期的过渡过程中，种子中GS的积累伴随着相应角果壁中GS的显著下降。因此，对这两个阶段的种子和相应的长角果壁进行转录组测序分析。共鉴定出135个GS代谢相关基因，其中24个为转录因子基因，81个为生物合成途径相关基因，25个为分解代谢酶基因，5个为转运蛋白基因。GS生物合成基因在种子和角果壁中都有表达。FMOGS-OX和AOP 2在两个时期的种子中都有高表达，这两个基因与侧修饰产生的拟南芥肽有关。有趣的是，GS生物合成基因在角果壁中的表达量高于种子，尽管角果壁中的GS含量低于种子。结合GTR基因在角果壁中的表达量高于种子中的表达量，推测GS从角果壁向种子的转运是种子GS积累的重要来源。此外，GS降解相关基因在子叶胚早期种子中大量表达，表明其在平衡种子GS含量方面具有潜在作用。结论鱼雷胚和子叶胚早期是种子发育过程中GS积累的关键时期。此外，我们证实了GS从角果壁运输到种子，并提出可能的GS生物合成的侧链修饰可能存在于种子形成过程中。
BackgroundTo understand the mechanism of glucosinolates (GSs) accumulation in the specific organs, combined analysis of physiological change and transcriptome sequencing were applied in the current study. Taking Chinese kale as material, seeds and silique walls were divided into different stages based on the development of the embryo in seeds and then subjected to GS analysis and transcriptome sequencing.ResultsThe main GS in seeds of Chinese kale were glucoiberin and gluconapin and their content changed with the development of the seed. During the transition of the embryo from torpedo- to the early cotyledonary-embryo stage, the accumulation of GS in the seed was accompanied by the salient decline of GS in the corresponding silique wall. Thus, the seed and corresponding silique wall at these two stages were subjected to transcriptomic sequencing analysis. 135 genes related to GS metabolism were identified, of which 24 genes were transcription factors, 81 genes were related to biosynthetic pathway, 25 genes encoded catabolic enzymes, and 5 genes matched with transporters. The expression of GS biosynthetic genes was detected both in seeds and silique walls. The high expression ofFMOGS-OXandAOP2, which is related to the production of gluconapin by side modification, was noted in seeds at both stages. Interestingly, the expression of GS biosynthetic genes was higher in the silique wall compared with that in the seed albeit lower content of GS existed in the silique wall than in the seed. Combined with the higher expression of transporter genesGTRsin silique walls than in seeds, it was proposed that the transportation of GS from the silique wall to the seed is an important source for seed GS accumulation. In addition, genes related to GS degradation expressed abundantly in the seed at the early cotyledonary-embryo stage indicating its potential role in balancing seed GS content.ConclusionsTwo stages including the torpedo-embryo and the early cotyledonary-embryo stage were identified as crucial in GS accumulation during seed development. Moreover, we confirmed the transportation of GS from the silique wall to the seed and proposed possible sidechain modification of GS biosynthesis may exist during seed formation.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
白光或红蓝光组合光周期对羽衣甘蓝芽苗的影响
影响因子：
5.6
作者：
Chen J;Chen Z;Li Z;Zhao Y;Chen X;Wang-Pruski G;Guo R
通讯作者：
Guo R
影响因子：
7.4
作者：
CHADCHAWAN, S;BISHOP, J;BRADLEY, D
通讯作者：
BRADLEY, D
影响因子：
7.7
作者：
Kissen, Ralph;Rossiter, John T.;Bones, Atle M.
通讯作者：
Bones, Atle M.
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影响因子：
9.2
作者：
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通讯作者：
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