Combined algicidal effect of urocanic acid, N-acetylhistamine and l-histidine to harmful alga Phaeocystis globosa.
Combined algicidal effect of urocanic acid, N-acetylhistamine and l-histidine to harmful alga Phaeocystis globosa.
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尿刊酸、N-乙酰组胺和L-组氨酸联合对有害藻球棕囊藻的杀藻作用
DOI：
10.1039/c8ra00749g
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发表时间：
2018-04-03
期刊：
影响因子：
3.9
通讯作者：
Yin, Pinghe
中科院分区：
文献类型：
--
作者：
Zhuang, Luer;Zhao, Ling;Yin, Pinghe
Bacillus sp. B1菌株对主要红潮藻类之一的Phaeocystis globosa产生的杀藻化合物经分离鉴定为尿酸（uro）、l-组氨酸（his）和n -乙酰组胺（ace）。96 h中位有效浓度EC50值为uro (8 μg mL−1)> ace (16 μg mL−1)>his （23 μg mL−1）的杀藻效果顺序。uro和ace的相互作用对球形褐囊藻具有协同作用，加速了其细胞内活性氧（ROS）水平的升高，并在96 h后进一步降低了抗氧化酶的活性，导致细胞膜完整性和细胞核结构的破坏。而另外两种二元混合物uro + his和ace + his对褐囊藻均有拮抗作用。ROS水平的升高表明藻细胞遭受了氧化损伤。过量的活性氧诱导丙二醛（MDA）含量升高，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）等抗氧化酶活性均在处理72 h后达到最大值。透射电镜（TEM）分析显示，这些含氮化合物破坏了细胞膜的完整性、叶绿体和细胞核结构。本研究将为研究各种杀藻化合物对有害藻球褐囊藻的联合抑藻作用提供有益的信息。本文首次研究了三种含氮化合物对有害藻类褐囊藻的单一和联合杀藻作用。
The algicidal compounds produced by Bacillus sp. strain B1 against Phaeocystis globosa, one of the main red-tide algae, were isolated and identified in a previous study as urocanic acid (uro), l-histidine (his) and N-acetylhistamine (ace). The 96 h median effective concentration EC50 values indicated the algicidal effect order of uro (8 μg mL−1) > ace (16 μg mL−1) > his (23 μg mL−1). The interaction between uro and ace had a synergistic effect on Phaeocystis globosa, accelerated the increase in its intracellular reactive oxygen species (ROS) levels, and further decreased the activities of antioxidases after 96 h, causing destruction of cell membrane integrity and nuclear structure. However, the other two binary mixtures uro + his and ace + his were both antagonistic to Phaeocystis globosa. The increase in the level of ROS indicated that the algal cells suffered from oxidative damage. The surplus ROS induced the increase in malondialdehyde (MDA) content and activities of antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT), all of which reached maxima after 72 h treatment. Transmission electron microscopy (TEM) analysis revealed that these nitrogen-containing compounds caused destruction of cell membrane integrity, chloroplasts and nuclear structure. The present study will provide useful information for the combined effect of algicidal compounds on the harmful alga Phaeocystis globosa. This is the first report to explore single and combined algicidal effects of three nitrogen-containing compounds against the harmful alga Phaeocystis globosa.
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影响因子：
4.1
作者：
BRODERIUS, SJ;KAHL, MD;HOGLUND, MD
通讯作者：
HOGLUND, MD
三角褐指菌上噬菌斑形成细菌 KD531 的裂解和趋化特性
影响因子：
5.2
作者：
Chen Z;Zheng W;Yang L;Boughner LA;Tian Y;Zheng T;Xu H
通讯作者：
Xu H
影响因子：
4.4
作者：
Lee, SO;Kato, J;Ohtake, H
通讯作者：
Ohtake, H
DOI：
10.1007/s002449900512
发表时间：
1999-08-01
影响因子：
4
作者：
Moreau, CJ;Klerks, PL;Haas, CN
通讯作者：
Haas, CN
影响因子：
2.8
作者：
Blauw, A. N.;Los, F. J.;Peperzak, L.
通讯作者：
Peperzak, L.