Gene expression analysis of forskolin treated basilar papillae identifies microRNA181a as a mediator of proliferation.
Gene expression analysis of forskolin treated basilar papillae identifies microRNA181a as a mediator of proliferation.
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DOI：
10.1371/journal.pone.0011502
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发表时间：
2010-07-09
期刊：
影响因子：
3.7
通讯作者：
Navaratnam DS
中科院分区：
文献类型：
--
作者：
Frucht CS;Uduman M;Duke JL;Kleinstein SH;Santos-Sacchi J;Navaratnam DS
鸟类的听觉毛细胞在受伤后会自发再生，而哺乳动物则不然。更好地了解鸟类毛细胞再生的分子事件可能有助于识别并最终操纵哺乳动物的相关途径，以刺激耳聋患者的毛细胞再生并恢复听力。使用 Affymetrix 全基因组鸡阵列在培养 24 (n = 6)、48 (n = 6) 和 72 (n = 12) 小时后对孵化后雏鸡的毛喉素处理（即增殖）和静止对照听觉上皮细胞进行基因表达分析。在毛喉素处理的上皮细胞中，许多被认为与细胞周期控制和内耳发育相关的基因显着上调（p<0.05；>两倍变化）。对数据进行基因集富集分析，识别出多种可能在再生组织中上调的 microRNA，包括 microRNA181a (miR181a)，已知它可以介导其他系统中的增殖。功能实验表明，通过 BrdU 掺入检测，miR181a 过表达足以刺激基底乳头内的增殖。此外，一些新产生的细胞表达早期毛细胞标记物肌球蛋白VI，表明miR181a转染可以导致新毛细胞的产生。这些研究发现了一种单一的 microRNA（miR181a），它可以引起鸡听觉上皮细胞增殖并产生新的毛细胞。
Auditory hair cells spontaneously regenerate following injury in birds but not mammals. A better understanding of the molecular events underlying hair cell regeneration in birds may allow for identification and eventually manipulation of relevant pathways in mammals to stimulate regeneration and restore hearing in deaf patients. Gene expression was profiled in forskolin treated (i.e., proliferating) and quiescent control auditory epithelia of post-hatch chicks using an Affymetrix whole-genome chicken array after 24 (n = 6), 48 (n = 6), and 72 (n = 12) hours in culture. In the forskolin-treated epithelia there was significant (p<0.05; >two-fold change) upregulation of many genes thought to be relevant to cell cycle control and inner ear development. Gene set enrichment analysis was performed on the data and identified myriad microRNAs that are likely to be upregulated in the regenerating tissue, including microRNA181a (miR181a), which is known to mediate proliferation in other systems. Functional experiments showed that miR181a overexpression is sufficient to stimulate proliferation within the basilar papilla, as assayed by BrdU incorporation. Further, some of the newly produced cells express the early hair cell marker myosin VI, suggesting that miR181a transfection can result in the production of new hair cells. These studies have identified a single microRNA, miR181a, that can cause proliferation in the chicken auditory epithelium with production of new hair cells.
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DOI：
10.1073/pnas.97.22.11722
发表时间：
2000-10-24
影响因子：
11.1
作者：
Baird, RA;Burton, MD;Naeger, RA
通讯作者：
Naeger, RA
DOI：
10.1111/j.1749-6632.1996.tb15693.x
发表时间：
1996-01-01
期刊：
NEW DIRECTIONS IN VESTIBULAR RESEARCH
影响因子：
--
作者：
Baird, RA;Steyger, PS;Schuff, NR
通讯作者：
Schuff, NR
影响因子：
9.8
作者：
Chang HY;Sneddon JB;Alizadeh AA;Sood R;West RB;Montgomery K;Chi JT;van de Rijn M;Botstein D;Brown PO
通讯作者：
Brown PO
影响因子：
2.5
作者：
Adler, HJ;Raphael, Y
通讯作者：
Raphael, Y
影响因子：
56.9
作者：
CORWIN, JT;COTANCHE, DA
通讯作者：
COTANCHE, DA