The intestinal microbiota and metabolites in patients with anorexia nervosa.
The intestinal microbiota and metabolites in patients with anorexia nervosa.
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DOI：
10.1080/19490976.2021.1902771
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发表时间：
2021-01
期刊：
影响因子：
12.2
通讯作者：
Papezova H
中科院分区：
文献类型：
--
作者：
Prochazkova P;Roubalova R;Dvorak J;Kreisinger J;Hill M;Tlaskalova-Hogenova H;Tomasova P;Pelantova H;Cermakova M;Kuzma M;Bulant J;Bilej M;Smitka K;Lambertova A;Holanova P;Papezova H
脑肠道微生物群的相互作用与各种神经和精神疾病有关。虽然神经性厌食症（AN）的病理生理学尚不完全清楚，但它可能与微生物群失调有关。我们的目的是阐明肠道微生物群在AN疾病病理生理学中的作用。我们分析了AN患者肠道微生物组（细菌组和真菌组）的组成和多样性，并将其与健康对照组进行比较。此外，各种神经递质和短链脂肪酸（SCFA）的水平分别通过MS和NMR分析粪便样本。生化，人体测量，心理测量资料进行了评估。细菌α多样性参数分析显示，在治疗前，AN患者的Chao 1指数仅增加，反映了其个体间的差异。随后，在AN患者中观察到核心微生物群耗竭迹象。AN患者中的OTU（操作分类单位）在分类学上属于Alistipes，Clostridiales，Christensenellaceae和Ruminococcaceae。AN患者中代表性不足的OTU是粪杆菌属、Agathastrin、拟杆菌属、布劳特氏菌属和毛螺菌属。与对照组相比，患者在肠道细菌组以及宏基因组含量方面表现出更大的个体间差异，表明细菌组功能发生了改变。与对照组相比，患者粪便样本中的血清素、GABA、多巴胺、丁酸盐和乙酸盐水平降低。真菌生物群系分析未显示AN患者和健康对照之间α多样性和真菌谱组成的显著差异，真菌组成与细菌谱也无任何相关性。我们的研究结果显示了重度AN患者肠道微生物组及其代谢产物的变化。虽然治疗性部分营养导致体重指数增加和心理测量参数改善，但SCFA和神经递质谱以及微生物群落组成在住院期间并未发生实质性变化，这可能是仅部分体重恢复引起的。
Brain-gut microbiota interactions are intensively studied in connection with various neurological and psychiatric diseases. While anorexia nervosa (AN) pathophysiology is not entirely clear, it is presumably linked to microbiome dysbiosis. We aimed to elucidate the gut microbiota contribution in AN disease pathophysiology. We analyzed the composition and diversity of the gut microbiome of patients with AN (bacteriome and mycobiome) from stool samples before and after renourishment, and compared them to healthy controls. Further, levels of assorted neurotransmitters and short-chain fatty acids (SCFA) were analyzed in stool samples by MS and NMR, respectively. Biochemical, anthropometric, and psychometric profiles were assessed. The bacterial alpha-diversity parameter analyses revealed only increased Chao 1 index in patients with AN before the realimentation, reflecting their interindividual variation. Subsequently, core microbiota depletion signs were observed in patients with AN. Overrepresented OTUs (operation taxonomic units) in patients with AN taxonomically belonged to Alistipes, Clostridiales, Christensenellaceae, and Ruminococcaceae. Underrepresented OTUs in patients with AN were Faecalibacterium, Agathobacter, Bacteroides, Blautia, and Lachnospira. Patients exhibited greater interindividual variation in the gut bacteriome, as well as in metagenome content compared to controls, suggesting altered bacteriome functions. Patients had decreased levels of serotonin, GABA, dopamine, butyrate, and acetate in their stool samples compared to controls. Mycobiome analysis did not reveal significant differences in alpha diversity and fungal profile composition between patients with AN and healthy controls, nor any correlation of the fungal composition with the bacterial profile. Our results show the changed profile of the gut microbiome and its metabolites in patients with severe AN. Although therapeutic partial renourishment led to increased body mass index and improved psychometric parameters, SCFA, and neurotransmitter profiles, as well as microbial community compositions, did not change substantially during the hospitalization period, which can be potentially caused by only partial weight recovery.
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影响因子：
3.7
作者：
Armougom F;Henry M;Vialettes B;Raccah D;Raoult D
通讯作者：
Raoult D
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
15.1
作者：
Jiang, Haiyin;Ling, Zongxin;Ruan, Bing
通讯作者：
Ruan, Bing
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM