Surface-enhanced Raman scattering of whole human blood, blood plasma, and red blood cells: cellular processes and bioanalytical sensing.
Surface-enhanced Raman scattering of whole human blood, blood plasma, and red blood cells: cellular processes and bioanalytical sensing.
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DOI：
10.1021/jp304932g
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发表时间：
2012-08-09
影响因子：
3.3
通讯作者：
Ziegler, L. D.
中科院分区：
文献类型：
--
作者：
Premasiri, W. R.;Lee, J. C.;Ziegler, L. D.
在785 nm激发下，观察到了人全血、血浆和红细胞在金纳米二氧化硅衬底上的SERS光谱。对于本文所用的样品制备程序，全血的SERS光谱仅来自血浆成分。这与全血在785 nm处激发并暴露在环境空气中的正常拉曼光谱形成鲜明对比，全血拉曼光谱完全是由于氧合血红蛋白的散射造成的。全血的SERS光谱显示出一种储存时间的依赖性，这在全血的非SERS拉曼光谱中并不明显。次黄嘌呤是嘌呤降解的产物，在8℃下保存约10-20小时后，次黄嘌呤在血液中的SERS谱占主导地位。从储存的血液中分离出的相应SERS谱显示，次黄嘌呤的时间释放相同。因此，血液细胞成分(红细胞、白细胞和/或血小板)在这段时间间隔内向血浆中释放次黄嘌呤。本文首次报道了红血球在785 nm激发下的表面增强拉曼光谱，发现了已知的血红素族标记谱带，以及其他可能与细胞膜成分或蛋白质变性有关的谱带。报道并比较了含氧和含MET的红细胞的表面增强拉曼光谱和正常拉曼光谱。这些SERS结果可以在临床诊断、血液供应管理和取证领域产生重大影响。
SERS spectra of whole human blood, blood plasma and red blood cells on Au nanoparticle SiO2 substrates excited at 785 nm have been observed. For the sample preparation procedure employed here, the SERS spectrum of whole blood arises from the blood plasma component only. This is in contrast to the normal Raman spectrum of whole blood excited at 785 nm and open to ambient air, which is exclusively due to the scattering of oxyhemoglobin. The SERS spectrum of whole blood shows a storage time dependence that is not evident in the non-SERS Raman spectrum of whole blood. Hypoxanthine, a product of purine degradation, dominates the SERS spectrum of blood after ~10 – 20 hours of storage at 8 °C. The corresponding SERS spectrum of plasma isolated from the stored blood shows the same temporal release of hypoxanthine. Thus, blood cellular components (red blood cells, white blood cells and/or platelets) are releasing hypoxanthine into the plasma over this time interval. The SERS spectrum of red blood cells (RBCs) excited at 785 nm is reported for the first time and exhibits well known heme group marker bands, as well as other bands that may be attributed to cell membrane components or protein denaturation contributions. SERS, as well as normal Raman spectra, of oxy- and met-RBCs are reported and compared. These SERS results can have significant impact in the area of clinical diagnostics, blood supply management and forensics.
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影响因子：
3.7
作者：
Bankapur A;Zachariah E;Chidangil S;Valiathan M;Mathur D
通讯作者：
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DOI：
10.1073/pnas.1016530108
发表时间：
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影响因子：
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DOI：
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发表时间：
2007-07-01
影响因子：
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作者：
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