The toxicity of ZnO nanomaterials to HepG2 cells: the influence of size and shape of particles
The toxicity of ZnO nanomaterials to HepG2 cells: the influence of size and shape of particles
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ZnO纳米材料对HepG2细胞的毒性：颗粒尺寸和形状的影响
DOI：
10.1002/jat.3712
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发表时间：
2018-08
影响因子：
3.3
通讯作者：
曹毅
中科院分区：
文献类型：
--
作者：
颜德健;龙继敏;刘冀锴;曹毅
了解物理化学性质在确定ZnO纳米材料（NMs）毒性中的可能作用对于ZnO基材料的安全使用至关重要。在这项研究中，我们合成了四种类型的ZnO纳米棒，并将其表征为ZnO纳米棒（NRs，长度400 ~ 500 nm，直径150 ~ 200 nm）， ZnO Mini - NRs（长度50 ~ 100 nm，直径15 ~ 20 nm），无定形ZnO微球（a‐ZnO MS）和晶体ZnO MS （c‐ZnO MS， a/c‐ZnO MS是广泛生长的片状结构的纳米花）。氧化锌NMs和氧化锌Mini‐NRs的细胞毒性明显高于a/c‐ZnO MS，这种趋势在HepG2细胞和人脐静脉内皮细胞中也相似。c‐ZnO MS仅能适度诱导细胞内活性氧，而所有类型的ZnO NMs均能使HepG2细胞内锌离子呈剂量依赖性增加。内质网应激标记物DDIT3的表达顺序为ZnO NRs > a‐ZnO MS > c‐ZnO MS > ZnO Mini‐NRs，凋亡基因CASP12的表达顺序为a‐ZnO MS > ZnO NRs > c‐ZnO MS > ZnO Mini‐NRs。综上所述，氧化锌纳米粒的大小和形状可能影响氧化锌纳米粒诱导的细胞毒性和内质网应激凋亡基因的表达。
Understanding the possible role of physicochemical properties in determining the toxicity of ZnO nanomaterials (NMs) is crucial for the safe use of ZnO‐based materials. In this study, we synthesized four types of ZnO NMs, and characterized them as ZnO nanorods (NRs; length 400‐500 nm, diameter 150‐200 nm), ZnO Mini‐NRs (length 50‐100 nm, diameter 15‐20 nm), amorphous ZnO microspheres (a‐ZnO MS) and crystalline ZnO MS (c‐ZnO MS; the a/c‐ZnO MS are nanoflowers with an extensive growth of sheet‐like structures). ZnO NMs and ZnO Mini‐NRs were significantly more cytotoxic than a/c‐ZnO MS, and this trend was similar in both HepG2 cells and human umbilical vein endothelial cells. Intracellular reactive oxygen species was only modestly induced by c‐ZnO MS, whereas intracellular Zn ions were dose‐dependently increased in HepG2 cells by the exposure of all types of ZnO NMs. The expression of endoplasmic reticulum stress marker DDIT3 was induced following an order of ZnO NRs > a‐ZnO MS > c‐ZnO MS > ZnO Mini‐NRs, and the apoptosis gene CASP12 was induced following an order of a‐ZnO MS > ZnO NRs > c‐ZnO MS > ZnO Mini‐NRs. Combined, these results suggested that ZnO NM‐induced cytotoxicity and expression of endoplasmic reticulum stress‐apoptosis genes could be influenced by the size and shape of ZnO NMs.
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影响因子：
12.4
作者：
An FF;Zhang XH
通讯作者：
Zhang XH
严重影响 ZnO 纳米颗粒毒性的多酚化学结构
影响因子：
6.1
作者：
Zhang Cao;Li Yining;Liu Liangliang;Gong Yu;Xie Yixi;Cao Yi
通讯作者：
Cao Yi
影响因子：
3.2
作者：
D. Shalini;S. Senthilkumar;P. Rajaguru
通讯作者：
D. Shalini;S. Senthilkumar;P. Rajaguru
影响因子：
3.5
作者：
Yang, Xia;Shao, Huali;Liu, Weirong;Gu, Weizhong;Shu, Xiaoli;Mo, Yiqun;Chen, Xuejun;Zhang, Qunwei;Jiang, Mizu
通讯作者：
Jiang, Mizu
影响因子：
5.1
作者：
Fang, Xin;Jiang, Leying;Cao, Yi
通讯作者：
Cao, Yi