Ion and water fluxes in the ileum of rats.
Ion and water fluxes in the ileum of rats.
复制标题
DOI：
10.1085/jgp.41.1.143
复制
发表时间：
1957-09-20
期刊：
影响因子：
--
通讯作者：
SOLOMON AK
中科院分区：
文献类型：
--
作者：
CURRAN PF;SOLOMON AK
已经对盐和水在大鼠小肠中的运动进行了研究。麻醉大鼠的回肠段已在体内灌注无缓冲的NaCl溶液或等渗溶液的NaCl和甘露醇。Na 24和Cl 36运动的动力学分析允许确定Na和Cl的流出和流入。使用血红蛋白作为参考物质测量净吸水率。人们发现水在渗透压梯度的作用下可以自由移动。等渗溶液中不同NaCl浓度的净水通量直接取决于净溶质通量。吸收的水量相当于将吸收的溶质维持在等渗浓度所需的量。这些结果已被解释为表明，水运动是一个被动的过程，取决于梯度的水活性和溶质的吸收速率。Na和Cl的流出是管腔中浓度的线性函数，但根据Ussing的标准（Acta Physiol.Scand.，1949，19，43）。管腔和等离子体之间的电位差已被解释为通过活性Cl通量超过活性Na通量的过量略微修改的扩散电位。上皮细胞膜的物理性质表明，它相当于一个膜，具有带负电荷的均匀的正圆孔，半径为36微米，占表面积的0.001%。
Studies have been carried out on the movement of salt and water across the small intestine of the rat. Segments of the ileum of anesthetized rats have been perfused in vivo with unbuffered NaCl solutions or isotonic solutions of NaCl and mannitol. Kinetic analysis of movements of Na24 and Cl36 has permitted determination of the efflux and influx of Na and Cl. Net water absorption has been measured using hemoglobin as a reference substance. Water was found to move freely in response to gradients of osmotic pressure. Net water flux from isotonic solutions with varying NaCl concentration was directly dependent on net solute flux. The amount of water absorbed was equivalent to the amount required to maintain the absorbed solute at isotonic concentration. These results have been interpreted as indicating that water movement is a passive process depending on gradients of water activity and on the rate of absorption of solute. The effluxes of Na and Cl are linear functions of concentration in the lumen, but both ions are actively transported by the ileum according to the criterion of Ussing (Acta Physiol. Scand., 1949, 19, 43). The electrical potential difference between the lumen and plasma has been interpreted as a diffusion potential slightly modified by the excess of active Cl flux over active Na flux. The physical properties of the epithelial membrane indicate that it is equivalent to a membrane having negatively charged uniform right circular pores of 36 Å radius occupying 0.001 per cent of the surface area.
登录
查看更多内容
影响因子：
3.8
作者：
DURBIN, RP;FRANK, H;SOLOMON, AK
通讯作者：
SOLOMON, AK
影响因子：
56.9
作者：
ROBINSON, CV
通讯作者：
ROBINSON, CV
影响因子：
--
作者：
BENSON, JA;LEE, PR;BOLLMAN, JL
通讯作者：
BOLLMAN, JL
影响因子：
15
作者：
HUNTER, MJ;COMMERFORD, SL
通讯作者：
COMMERFORD, SL
影响因子：
15.9
作者：
DAGOSTINO, A;LEADBETTER, WF;SCHWARTZ, WB
通讯作者：
SCHWARTZ, WB