Instantaneous 4D micro-particle image velocimetry (µPIV) via multifocal microscopy (MUM).
Instantaneous 4D micro-particle image velocimetry (µPIV) via multifocal microscopy (MUM).
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DOI：
10.1038/s41598-022-22701-3
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发表时间：
2022-11-02
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
多焦显微镜（MUM）是一种同时在一台相机上从不同轴向面捕获多个视场（fov）的技术，用于执行微粒子图像测速（µPIV），以重建液体在细胞周围流动时施加的速度和剪切应力场。采用基于衍射的多焦继电器在轴距为630 nm的三个不同平面上采集图像，利用图像清晰度度量计算出流示踪颗粒的轴向位置。结果表明，MUM在计算速度上的精度约为（0.52±0.19）µm/s。使用固定细胞，MUM对亚细胞水平的流动扰动进行成像，其特征与文献中观察到的相似。MUM以活细胞为例，观察了灌注过程中细胞形态变化对局部血流的影响。与标准共聚焦激光扫描显微镜相比，MUM在µPIV的采集速度上具有明显的优势（超过300倍）。这是快速进化的生物系统的一个重要特征，其中有必要实时监测整个体积以将样品响应与外力相关联。
Multifocal microscopy (MUM), a technique to capture multiple fields of view (FOVs) from distinct axial planes simultaneously and on one camera, was used to perform micro-particle image velocimetry (µPIV) to reconstruct velocity and shear stress fields imposed by a liquid flowing around a cell. A diffraction based multifocal relay was used to capture images from three different planes with 630 nm axial spacing from which the axial positions of the flow-tracing particles were calculated using the image sharpness metric. It was shown that MUM can achieve an accuracy on the calculated velocity of around (0.52 ± 0.19) µm/s. Using fixed cells, MUM imaged the flow perturbations at sub-cellular level, which showed characteristics similar to those observed in the literature. Using live cells as an exemplar, MUM observed the effect of changing cell morphology on the local flow during perfusion. Compared to standard confocal laser scanning microscope, MUM offers a clear advantage in acquisition speed for µPIV (over 300 times faster). This is an important characteristic for rapidly evolving biological systems where there is the necessity to monitor in real time entire volumes to correlate the sample responses to the external forces.
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影响因子：
16.1
作者：
Lynch ME;Fischbach C
通讯作者：
Fischbach C
影响因子：
48
作者：
Abrahamsson, Sara;Chen, Jiji;Hajj, Bassam;Stallinga, Sjoerd;Katsov, Alexander Y.;Wisniewski, Jan;Mizuguchi, Gaku;Soule, Pierre;Mueller, Florian;Darzacq, Claire Dugast;Darzacq, Xavier;Wu, Carl;Bargmann, Cornelia I.;Agard, David A.;Dahan, Maxime;Gustafsson, Mats G. L.
通讯作者：
Gustafsson, Mats G. L.
影响因子：
--
作者：
Ujihara Y;Nakamura M;Miyazaki H;Wada S
通讯作者：
Wada S
DOI：
10.7171/jbt.15-2602-003
发表时间：
2015-07-01
期刊：
Journal of biomolecular techniques : JBT
影响因子：
--
作者：
Jonkman, James;Brown, Claire M
通讯作者：
Brown, Claire M
影响因子：
5.3
作者：
Docheva D;Padula D;Popov C;Mutschler W;Clausen-Schaumann H;Schieker M
通讯作者：
Schieker M