IL-10 to TNFα ratios throughout early first trimester can discriminate healthy pregnancies from pregnancy losses.
IL-10 to TNFα ratios throughout early first trimester can discriminate healthy pregnancies from pregnancy losses.
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DOI：
10.1111/aji.13195
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发表时间：
2020-01
期刊：
影响因子：
--
通讯作者：
Mor G
中科院分区：
文献类型：
--
作者：
Kaislasuo J;Simpson S;Petersen JF;Peng G;Aldo P;Lokkegaard E;Paidas M;Pal L;Guller S;Mor G
胚胎植入和胎盘形成需要仔细的免疫平衡。细胞因子，如IL-10和肿瘤坏死因子α，已被认为是调节失调的标志，但仅在单个时间点或在妊娠丢失后进行研究。我们的目标是确定健康妊娠早期血清IL-10和肿瘤坏死因子α水平及其比率的标准模式，并确定这种模式是否与妊娠丢失不同。包括体外受精(IVF)和连续抽血自然受孕的两个预期纵向队列。用Simple Plex软件检测细胞因子。在试管受精队列中，我们从植入之日起一直监测到妊娠6周；而在自然受孕的队列中，样本收集从4周开始，并贯穿整个妊娠早期。正常妊娠组IL-10浓度显著高于妊娠6-8周开始丢失的孕妇，而肿瘤坏死因子α的浓度显著低于妊娠3-5周开始丢失的孕妇。正常妊娠4~9周的IL-10/肿瘤坏死因子α比值显著高于流产孕妇(t检验，P<；0.05)。在出现任何流产症状之前就观察到了变化。我们提供了健康妊娠的早期免疫调节与那些注定要在妊娠早期丢失的妊娠早期免疫调节不同的证据。IL-10与肿瘤坏死因子α的比值在妊娠早期4.5wk时显著高于丢失妊娠组。
Embryo implantation and placentation require a careful immunological balance. Cytokines such as IL-10 and TNFα have been implicated as markers of dysregulation, but have only been studied at a single time point or after a pregnancy loss. Our objective was to determine normative patterns of serum levels of IL-10 and TNFα and their ratio throughout the first trimester in healthy pregnancies and to determine if this pattern differs from pregnancy loss. Two prospective longitudinal cohorts of gravidae including in vitro fertilization (IVF) and naturally conceived pregnancies with serial blood draws. Cytokines were assayed using Simple Plex. In the IVF cohort, we monitored from the implantation day up to 6 weeks of gestation; whereas in the naturally conceived cohort, sample collection began at 4 weeks and throughout the whole first trimester. IL-10 concentrations in normal pregnancies were significantly higher than in pregnancies ending in a loss starting at 6-8 weeks of gestation, while TNFα concentrations were significantly lower in normal than in pregnancies ending in a loss starting at 3-5 of gestation weeks. The IL-10 to TNFα ratio in normal pregnancies was significantly higher from 4 to 9 weeks compared to pregnancies that were lost (t test, P < .05). Changes were observed before any symptoms of miscarriage were present. We provide evidences of differences in early immunomodulation in healthy pregnancies vs those destined to end in first-trimester loss. The ratio of IL-10 to TNFα rises significantly higher in viable pregnancies as early as 4.5 weeks compared to pregnancies loss.
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影响因子：
5.2
作者：
Mor G;Cardenas I;Abrahams V;Guller S
通讯作者：
Guller S
DOI：
10.1111/j.1600-0897.2009.00792.x
发表时间：
2010-01
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
--
作者：
Dekel N;Gnainsky Y;Granot I;Mor G
通讯作者：
Mor G
影响因子：
6.7
作者：
Jenkins, C;Roberts, J;Walker, JJ
通讯作者：
Walker, JJ
DOI：
10.1111/aji.12512
发表时间：
2016-06
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
--
作者：
Aldo P;Marusov G;Svancara D;David J;Mor G
通讯作者：
Mor G
影响因子：
6.1
作者：
Boomsma, C. M.;Kavelaars, A.;Macklon, N. S.
通讯作者：
Macklon, N. S.