A novel in vitro analog expressing learning-induced cellular correlates in distinct neural circuits.
A novel in vitro analog expressing learning-induced cellular correlates in distinct neural circuits.
复制标题
DOI：
10.1101/lm.045229.117
复制
发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Mozzachiodi R
中科院分区：
文献类型：
--
作者：
Weisz HA;Wainwright ML;Mozzachiodi R
当受到有害刺激时，海蛾表现出同步的防御反应敏化，如尾巴诱导的虹吸退缩反射（TSWR）和进食抑制。在细胞水平上，TSWR的敏化伴随着引起反射的尾部感觉神经元（tsn）兴奋性的增加，而摄食抑制伴随着摄食神经回路中决策神经元B51的兴奋性降低。本研究的目的是开发一种体外共表达上述细胞相关因子的类似物。我们使用了由颊神经节、脑神经节和胸膜足神经节组成的简化制剂，它们分别包含控制摄食和TSWR的神经回路。在体外通过电刺激传入神经来传递致敏刺激。当接受致敏刺激训练时，体外类似物同时表达tsn的兴奋性增加和B51的兴奋性降低，这与体内训练诱导的致敏和摄食抑制的发生一致。这种体外模拟物表达了tsn和B51的短期（15分钟）和长期（24小时）兴奋性变化，这取决于所给予的训练量。最后，在体外应用5 -羟色胺增加TSN的兴奋性，但不改变B51的兴奋性，反映了体内应用单胺诱导致敏，但不抑制喂养。
When presented with noxious stimuli, Aplysia exhibits concurrent sensitization of defensive responses, such as the tail-induced siphon withdrawal reflex (TSWR) and suppression of feeding. At the cellular level, sensitization of the TSWR is accompanied by an increase in the excitability of the tail sensory neurons (TSNs) that elicit the reflex, whereas feeding suppression is accompanied by decreased excitability of B51, a decision-making neuron in the feeding neural circuit. The goal of this study was to develop an in vitro analog coexpressing the above cellular correlates. We used a reduced preparation consisting of buccal, cerebral, and pleural-pedal ganglia, which contain the neural circuits controlling feeding and the TSWR, respectively. Sensitizing stimuli were delivered in vitro by electrical stimulation of afferent nerves. When trained with sensitizing stimuli, the in vitro analog expressed concomitant increased excitability in TSNs and decreased excitability in B51, which are consistent with the occurrence of sensitization and feeding suppression induced by in vivo training. This in vitro analog expressed both short-term (15 min) and long-term (24 h) excitability changes in TSNs and B51, depending on the amount of training administered. Finally, in vitro application of serotonin increased TSN excitability without altering B51 excitability, mirroring the in vivo application of the monoamine that induces sensitization, but not feeding suppression.
登录
查看更多内容
影响因子：
7.2
作者：
Byrne, John H.;Hawkins, Robert D.
通讯作者：
Hawkins, Robert D.
影响因子：
5.3
作者：
Antonov, Igor;Ha, Thomas;Hawkins, Robert D.
通讯作者：
Hawkins, Robert D.
影响因子：
56.9
作者：
Brembs, B;Lorenzetti, FD;Byrne, JH
通讯作者：
Byrne, JH
影响因子：
2.7
作者：
Dickinson, Kathy J.;Wainwright, Marcy L.;Mozzachiodi, Riccardo
通讯作者：
Mozzachiodi, Riccardo
影响因子：
16.6
作者：
Crossley M;Staras K;Kemenes G
通讯作者：
Kemenes G