Recombination form and epidemiology of HIV-1 unique recombinant strains identified in Yunnan, China.
Recombination form and epidemiology of HIV-1 unique recombinant strains identified in Yunnan, China.
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中国云南鉴定出的HIV-1独特重组株的重组形式和流行病学
DOI：
10.1371/journal.pone.0046777
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Li L;Chen L;Yang S;Li T;Li J;Liu Y;Jia L;Yang B;Bao Z;Li H;Wang X;Zhuang D;Liu S;Li J
几项研究在中国云南的一些不同地区发现了HIV-1重组。但是，到目前为止，还没有在全省范围内进行全面的研究。为了解云南省HIV-1独特重组体（Unique Recombinant Forms，URF）的流行情况和重组形式，随机抽取云南省15个地（市）788名HIV-1阳性者进行研究。扩增全长gag和pol基因并测序。构建最大似然树进行系统发育分析。用在线软件jpHMM鉴定重组断裂点和基因组示意图。在617株具有亚型的菌株中鉴定出63株（10.2%）独特的重组菌株。各地市之间的URF分布差异有统计学意义（Pearson卡方检验，P<0.05）。静脉吸毒人群URF检出率高于性传播人群（Pearson卡方检验，P<0.05）。根据全长gag-pol基因中CRF 01-AE片段的存在，鉴定了两种主要的重组体：B′/C和B′/C/CRF 01-AE重组体。在ML树中识别出三个簇，其包含三个以上的序列并且由高自举值支持。确定了一份CRF。许多统一报告格式载有相同的断点。本研究结果将有助于我们对HIV重组的理解，并为中国潜在CRF的鉴定提供线索。
Several studies identified HIV-1 recombination in some distinct areas in Yunnan, China. However, no comprehensive studies had been fulfilled in the whole province up to now. To illustrate the epidemiology and recombination form of Unique Recombinant Forms (URFs) circulating in Yunnan, 788 HIV-1 positive individuals residing in 15 prefectures of Yunnan were randomly enrolled into the study. Full-length gag and pol genes were amplified and sequenced. Maximum likelihood tree was constructed for phylogenetic analysis. Recombinant breakpoints and genomic schematics were identified with online software jpHMM. 63 (10.2%) unique recombinant strains were identified from 617 strains with subtypes. The URFs distributed significantly differently among prefectures (Pearson chi-square test, P<0.05). IDUs contained more URFs than sexual transmitted population (Pearson chi-square test, P<0.05). Two main recombinant forms were identified by considering the presence of CRF01_AE segments in full length gag-pol genes, which were B′/C and B′/C/CRF01-AE recombinants. Three clusters were identified in the ML tree which contained more than three sequences and supported by high bootstrap values. One CRF was identified. Many of URFs contained identical breakpoints. The results will contribute to our understanding on HIV recombination and provide clues to the identification of potential CRFs in China.
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DOI：
10.1097/qad.0b013e328342ff93
发表时间：
2011-03-13
期刊：
AIDS (London, England)
影响因子：
--
作者：
Hemelaar J;Gouws E;Ghys PD;Osmanov S;WHO-UNAIDS Network for HIV Isolation and Characterisation
通讯作者：
WHO-UNAIDS Network for HIV Isolation and Characterisation
影响因子：
3.2
作者：
Li, Lin;Lu, Xinli;Li, Jingyun
通讯作者：
Li, Jingyun
影响因子：
5.4
作者：
Sagar, Manish;Wu, Xueling;Overbaugh, Julie
通讯作者：
Overbaugh, Julie
影响因子：
3.8
作者：
Takebe, Y;Motomura, K;Kusagawa, S
通讯作者：
Kusagawa, S
影响因子：
3.8
作者：
XIA, MS;KREISS, JK;HOLMES, KK
通讯作者：
HOLMES, KK