Curcumin-loaded poly(epsilon-caprolactone) nanofibres: diabetic wound dressing with anti-oxidant and anti-inflammatory properties.
Curcumin-loaded poly(epsilon-caprolactone) nanofibres: diabetic wound dressing with anti-oxidant and anti-inflammatory properties.
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DOI：
10.1111/j.1440-1681.2009.05216.x
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发表时间：
2009-12
影响因子：
2.9
通讯作者：
Nair LS
中科院分区：
文献类型：
--
作者：
Merrell JG;McLaughlin SW;Tie L;Laurencin CT;Chen AF;Nair LS
1.姜黄素是一种天然存在的多酚类化合物，具有广泛的生物学功能，包括抗癌、抗氧化和抗炎活性。姜黄素的低生物利用度和体内稳定性需要开发合适的载体媒介物以治疗水平以持续的方式递送分子。2.我们研究了聚己内酯（PCL）纳米纤维作为姜黄素用于伤口愈合应用的递送载体的可行性和潜力。通过优化静电纺丝工艺参数，制备了载姜黄素的无珠PCL纳米纤维。3.该纤维显示出持续释放姜黄素72小时，并可以提供比报告的细胞毒性浓度低得多的剂量，同时保持生物活性。人包皮成纤维细胞（HFF-1）在负载姜黄素的纳米纤维上显示出超过70%的活力。负载姜黄素的纳米纤维的抗氧化活性使用ORAC测定和通过纤维在氧化应激条件下维持纤维上的HFF-1细胞的活力的能力来证明。负载姜黄素的纳米纤维还减少了炎症诱导，如通过在大肠杆菌衍生的脂多糖（LPS）刺激后接种在纤维上的小鼠单核细胞-巨噬细胞的低水平IL-6释放所证明的。负载姜黄素的PCL纳米纤维的体内伤口愈合能力通过链脲佐菌素（STZ）诱导的糖尿病小鼠模型中伤口闭合速率的增加来证明。4.这些结果表明，负载姜黄素的PCL基质是生物活性的，并且具有作为具有抗氧化和抗炎性质的伤口敷料的潜力。
1. Curcumin is a naturally occurring poly-phenolic compound with a broad range of favorable biological functions including anti-cancer, anti-oxidant and anti-inflammatory activities. The low bioavailability and in vivo stability of curcumin require the development of suitable carrier vehicles to deliver the molecule in a sustained manner at therapeutic levels. 2. We investigated the feasibility and potential of poly(caprolactone) (PCL) nanofibers as a delivery vehicle for curcumin for wound healing applications. By optimizing the electrospinning parameters, bead-free curcumin loaded PCL nanofibers were developed. 3. The fibers showed sustained release of curcumin for 72 h and could be made to deliver a dosage much lower than the reported cytotoxic concentration while remaining bioactive. Human foreskin fibroblast cells (HFF-1) showed more than 70% viability on curcumin loaded nanofibers. The antioxidant activity of curcumin loaded nanofibers was demonstrated using an ORAC assay and by the ability of the fibers to maintain the viability of HFF-1 cells on the fibers under a condition of oxidative stress. The curcumin loaded nanofibers also reduced inflammatory induction as evidenced by low levels of IL-6 release from mouse monocyte-macrophages seeded on the fibers following stimulation by E.coli-derived lipopolysaccharide (LPS). The in vivo wound healing capability of the curcumin loaded PCL nanofibers was demonstrated by an increased rate of wound closure in a streptozotocin (STZ) induced diabetic mice model. 4. These results demonstrate that curcumin loaded PCL nanofiber matrix is bioactive and has potential as a wound dressing with antioxidant and anti-inflammatory properties.
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影响因子：
5
作者：
Notoya, M;Nishimura, H;Hagiwara, H
通讯作者：
Hagiwara, H
影响因子：
4.5
作者：
Kowluru RA;Kanwar M
通讯作者：
Kanwar M
影响因子：
10.2
作者：
Bisht S;Feldmann G;Soni S;Ravi R;Karikar C;Maitra A;Maitra A
通讯作者：
Maitra A
影响因子：
4.3
作者：
Panchatcharam, Manikandan;Miriyala, Sumitra;Suguna, Lonchin
通讯作者：
Suguna, Lonchin
影响因子：
14
作者：
Fujihara, K;Kotaki, M;Ramakrishna, S
通讯作者：
Ramakrishna, S