Serotoninergic differentiation of quail neural crest cells in vitro.
Serotoninergic differentiation of quail neural crest cells in vitro.
复制标题
鹌鹑神经嵴细胞的体外血清素能分化。
DOI：
10.1016/0012-1606(83)90285-3
复制
发表时间：
1983
影响因子：
2.7
通讯作者：
Lidov,HG
中科院分区：
文献类型：
--
作者：
Sieber-Blum,M;Reed,W;Lidov,HG
本文报道了在体外条件下向5-羟色胺能神经元分化的方法。用甲醛诱导的组织荧光法和抗5-羟色胺抗体的间接染色两种独立的方法鉴定5-羟色胺能神经元。5-羟色胺阳性细胞最早出现在培养的第3天，与第一批着色细胞和肾上腺素能神经元同时出现或稍早出现。同样数量的5-羟色胺能细胞在来源于迷走神经、胸椎/上腰椎和腰椎神经轴水平的脊细胞培养中被发现。5-羟色胺神经元的神经脊来源进一步得到证实：体节、脊索和神经管的培养(与神经脊相邻的三个组织，因此最有可能是5-羟色胺细胞培养物的污染物)不含有产生5-羟色胺的细胞，并且在脊细胞培养中不存在肥大细胞。对培养的5-羟色胺能神经元进行鉴定，可以增加培养中能够分化的神经脊衍生物的数量。此外，本研究还表明，吴登盛的体外培养体系将被证明是阐明支配神经脊细胞分化的细胞和分子机制的有效途径。
Thein vitrodifferentiation of quail neural crest cells into serotoninergic neurons is reported. Serotoninergic neurons were identified by two independent methods, formaldehyde-induced histofluorescence and indirect staining with antiserotonin antibodies. Serotonin-positive cells first appeared on the third day in culture, simultaneously, or slightly prior to the first pigmented cells and adrenergic neurons. Comparable numbers of serotoninergic cells were found in crest cell cultures derived from vagal, thoracic/upper lumbar, and lumbosacral levels of the neuraxis. The neural crest origin of the serotonin neurons was further corroborated by the demonstration that cultures of somites, notochords, and neural tubes (three tissues adjacent to the neural crest and thus the most likely contaminants of crest cell cultures) did not contain serotonin-producing cells, and that mast cells were absent in crest cell cultures. The identification of serotoninergic neurons in quail neural crest cell cultures makes an important addition to the number of neural crest derivatives that are capable of differentiating in culture. Furthermore, it suggests that thein vitroculture system will prove a valid approach to the elucidation of the cellular and molecular mechanisms that govern neural crest cell differentiation.
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促肿瘤佛波酯促进黑色素生成并阻止鹌鹑神经嵴细胞中肾上腺素能表型的表达。
DOI：
10.1111/j.1432-0436.1981.tb01165.x
发表时间：
1981
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
Sieber-Blum,M;Sieber,F
通讯作者：
Sieber,F
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影响因子：
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作者：
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通讯作者：
N. Douarin
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影响因子：
2.9
作者：
A. Liuzzi;F. H. Foppen;J. Saavedra;Rita Levi;I. Kopin
通讯作者：
I. Kopin
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影响因子：
2.9
作者：
T. P. Rothman;L. Ross;M. Gershon
通讯作者：
M. Gershon
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影响因子：
2.7
作者：
M. Sieber;A. M. Cohen
通讯作者：
A. M. Cohen