Hemocompatibility assessment of carbonic anhydrase modified hollow fiber membranes for artificial lungs.
Hemocompatibility assessment of carbonic anhydrase modified hollow fiber membranes for artificial lungs.
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DOI：
10.1111/j.1525-1594.2009.00882.x
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发表时间：
2010-05
影响因子：
2.4
通讯作者：
Wagner WR
中科院分区：
文献类型：
--
作者：
Oh HI;Ye SH;Johnson CA Jr;Woolley JR;Federspiel WJ;Wagner WR
基于中空纤维膜（HFM）的人工肺可能需要大的血液接触膜表面积以提供足够的气体交换。然而，如此大的表面积对血液相容性提出了重大挑战。提高二氧化碳（CO2）转移效率的一种方法可能是将碳酸酐酶（CA）固定到常规HFM的表面上。通过催化血液中碳酸氢盐的脱水，CA已被证明有助于CO2向纤维膜的扩散。本研究评价了表面改性的市售微孔HFM基人工肺对纤维血液生物相容性的影响。商业聚（丙烯）Celgard HFM表面涂覆有硅氧烷，接枝有胺基，然后与CA连接，这已被证明有助于CO2向纤维膜的扩散。急性绵羊血液接触后的结果表明，相对于基础HFM，硅氧烷涂层或具有接枝胺的硅氧烷涂层的血小板沉积或活化没有显著减少。然而，与所有其他纤维类型相比，附着CA的HFM在血小板沉积和活化方面均显着减少。这些发现，沿着在CA改性纤维中观察到的改善的CO2转移，表明将其并入HFM设计中可能会增强更小、更生物相容性的基于HFM的人工肺的设计。
Hollow fiber membrane (HFM)-based artificial lungs can require a large blood-contacting membrane surface area to provide adequate gas exchange. However, such a large surface area presents significant challenges to hemocompatibility. One method to improve carbon dioxide (CO2) transfer efficiency might be to immobilize carbonic anhydrase (CA) onto the surface of conventional HFMs. By catalyzing the dehydration of bicarbonate in blood, CA has been shown to facilitate diffusion of CO2 toward the fiber membranes. This study evaluated the impact of surface modifying a commercially available microporous HFM-based artificial lung on fiber blood biocompatibility. A commercial poly(propylene) Celgard HFM surface was coated with a siloxane, grafted with amine groups, and then attached with CA which has been shown to facilitate diffusion of CO2 toward the fiber membranes. Results following acute ovine blood contact indicated no significant reduction in platelet deposition or activation with the siloxane coating or the siloxane coating with grafted amines relative to base HFMs. However,HFMs with attached CA showed a significant reduction in both platelet deposition and activation compared with all other fiber types. These findings, along with the improved CO2 transfer observed in CA modified fibers, suggest that its incorporation into HFM design may potentiate the design of a smaller, more biocompatible HFM-based artificial lung.
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影响因子：
4.2
作者：
Eash, HJ;Jones, HM;Federspiel, WJ
通讯作者：
Federspiel, WJ
影响因子：
2.4
作者：
Johnson, Carl A., Jr.;Snyder, Trevor A.;Wagner, William R.
通讯作者：
Wagner, William R.
影响因子：
4.9
作者：
Ye, Sang-Ho;Johnson, Carl A.;Woolley, Joshua R.;Snyder, Trevor A.;Gamble, Lara J.;Wagner, William R.
通讯作者：
Wagner, William R.
影响因子：
14
作者：
Liu, TY;Lin, WC;Yang, MC
通讯作者：
Yang, MC
影响因子：
38.9
作者：
Conrad, SA;Zwischenberger, JB;Bidani, A
通讯作者：
Bidani, A