Chronic stress and a cyclic regimen of estradiol administration separately facilitate spatial memory: relationship with hippocampal CA1 spine density and dendritic complexity.
Chronic stress and a cyclic regimen of estradiol administration separately facilitate spatial memory: relationship with hippocampal CA1 spine density and dendritic complexity.
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DOI：
10.1037/a0025770
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发表时间：
2012-02
影响因子：
1.9
通讯作者：
Sparks M
中科院分区：
文献类型：
--
作者：
Conrad CD;McLaughlin KJ;Huynh TN;El-Ashmawy M;Sparks M
本研究探讨了慢性约束应激和重复循环雌二醇脉冲对雌性大鼠海马CA3和CA1树突和/或脊柱形态和空间记忆的影响。Sprague-Dawley成年雌性大鼠切除卵巢，注射17β-雌二醇（10µg, s.c） 2天，每4-5天重复一次。虽然所有大鼠都接受了类似的雌二醇注射史，但在进行海马依赖物体放置（OP）任务评估空间记忆之前，一半的大鼠被长期限制和/或给予雌二醇的最后循环脉冲。OP测试在最后一次约束后两天进行，也是最后两次雌二醇脉冲对海马CA1脊柱密度影响最大的时候。这些数据揭示了一些新的发现：1)慢性应激或雌二醇分别促进空间记忆，但在共同给药时没有相同的效果；2)CA1脊柱密度与空间记忆负相关；3)重复雌二醇脉冲不能防止应激诱导的CA3树突退缩。我们也证实了先前的研究表明，在雌二醇、慢性应激和行为操作后，CA1脊柱密度增加。本研究独特地结合了慢性应激、重复雌二醇脉冲、海马形态和同一动物的行为，允许在CA1脊柱形态和空间记忆之间进行相关性分析。我们证明了新的发现，慢性应激或雌二醇脉冲单独促进空间记忆，但不是当共同给予时，这些影响可能涉及CA1根尖脊柱表达的平衡，独立于CA3树突复杂性。
This study investigated the effects of chronic restraint stress and repeated cyclic estradiol pulses on hippocampal CA3 and CA1 dendritic and/or spine morphology and spatial memory in female rats. Sprague-Dawley adult female rats were ovariectomized and then injected over two days with 17β-estradiol (10µg, s.c.), which was repeated every 4–5 days. While all rats received similar estradiol injection histories, half of the rats were chronically restrained and/or given a final cyclic pulse of estradiol prior to testing on a hippocampal-dependent object placement (OP) task to assess spatial memory. OP testing was performed two days after the last restraint session, as well as when the last two estradiol pulses best captured the maximal effect on hippocampal CA1 spine density. The data revealed several novel findings: 1) chronic stress or estradiol separately facilitated spatial memory, but did not have the same effects when co-administered, 2) CA1 spine densities negatively correlated with spatial memory, and 3) repeated estradiol pulses failed to prevent stress-induced CA3 dendritic retraction. We also corroborated previous studies showing increased CA1 spine density following estradiol, chronic stress, and behavioral manipulations. The present study uniquely combined chronic stress, repeated estradiol pulses, hippocampal morphology and behavior within the same animals, allowing for correlational analyses to be performed between CA1 spine morphology and spatial memory. We demonstrate novel findings that chronic stress or estradiol pulses independently facilitate spatial memory, but not when co-administered, and that these effects may involve a balance of CA1 apical spine expression that is independent of CA3 dendritic complexity.
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影响因子：
2.9
作者：
Beck, KD;Luine, VN
通讯作者：
Luine, VN
影响因子：
4.2
作者：
Gibbs, RB
通讯作者：
Gibbs, RB
影响因子：
4.8
作者：
Daniel, JM;Hulst, JL;Berbling, JL
通讯作者：
Berbling, JL
影响因子：
2.7
作者：
Conrad, CD;Grote, KA;Ferayorni, A
通讯作者：
Ferayorni, A
影响因子：
2.7
作者：
Ainge, JA;Heron-Maxwell, C;Wood, ER
通讯作者：
Wood, ER