Serotonin receptor activity is necessary for olfactory learning and memory in Drosophila melanogaster.
Serotonin receptor activity is necessary for olfactory learning and memory in Drosophila melanogaster.
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DOI：
10.1016/j.neuroscience.2011.06.058
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发表时间：
2011-09-29
期刊：
影响因子：
3.3
通讯作者：
Nichols, C. D.
中科院分区：
文献类型：
--
作者：
Johnson, O.;Becnel, J.;Nichols, C. D.
果蝇的学习和记忆是一种复杂的行为，与哺乳动物的学习和记忆有很多相似之处。尽管包括乙酰胆碱、多巴胺、谷氨酸和 GABA 在内的许多神经递质先前已被证明与厌恶性嗅觉学习和记忆有关，但血清素的作用尚未明确。在这里，我们提出了个体血清素受体参与果蝇嗅觉学习和记忆的第一个证据。我们最初采用药理学方法，利用血清素受体激动剂和拮抗剂来证明果蝇中存在的所有血清素受体家族对于短期学习和记忆都是必需的。利用药物组合的等辐射分析揭示了 5-HT1A 样和 5-HT2 以及 5-HT2 和 5-HT7 受体回路之间发生功能相互作用，以调节短期学习和记忆。长期记忆检查表明，5-HT1A 样受体对于巩固和回忆很重要，5-HT2 受体对于巩固和回忆很重要，5-HT7 受体参与所有三个阶段。重要的是，我们通过基因实验验证了我们的药理学结果，并表明 5-HT2Dro 和 5-HT1BDro 受体的亚型菌株以及 5-HT7Dro mRNA 的敲低会显着损害短期记忆的表现。我们的数据强调了血清素系统和个体血清素受体对影响果蝇嗅觉学习和记忆的重要性，并将果蝇定位为模型系统来研究血清素在与哺乳动物中枢神经系统功能相关的认知过程中的作用。
Learning and memory in the fruit fly, Drosophila melanogaster, is a complex behavior with many parallels to mammalian learning and memory. Although many neurotransmitters including acetylcholine, dopamine, glutamate, and GABA have previously been demonstrated to be involved in aversive olfactory learning and memory, the role of serotonin has not been well defined. Here, we present the first evidence of the involvement of individual serotonin receptors in olfactory learning and memory in the fly. We initially followed a pharmacological approach, utilizing serotonin receptor agonists and antagonists to demonstrate that all serotonin receptor families present in the fly are necessary for short term learning and memory. Isobolographic analysis utilizing combinations of drugs revealed functional interactions are occurring between 5-HT1A-like and 5-HT2, and 5-HT2 and 5-HT7 receptor circuits in mediating short term learning and memory. Examination of long term memory suggest that 5-HT1A-like receptors are necessary for consolidation and important for recall, 5-HT2 receptors are important for consolidation and recall, and 5-HT7 receptors are involved in all three phases. Importantly, we have validated our pharmacological results with genetic experiments, and show that hypomorph strains for 5-HT2Dro and 5-HT1BDro receptors, as well as knockdown of 5-HT7Dro mRNA significantly impair performance in short term memory. Our data highlight the importance of the serotonin system and individual serotonin receptors to influence olfactory learning and memory in the fly, and position the fly as a model system to study the role of serotonin in cognitive processes relevant to mammalian CNS function.
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影响因子：
10.6
作者：
Lucki, I
通讯作者：
Lucki, I
影响因子：
3.7
作者：
Becnel J;Johnson O;Luo J;Nässel DR;Nichols CD
通讯作者：
Nichols CD
扇形体和椭球体在果蝇视觉模式记忆中的不同作用
影响因子：
2
作者：
Pan, Yufeng;Zhou, Yanqiong;Liu, Li
通讯作者：
Liu, Li
影响因子：
3.3
作者：
Nichols, CD;Ronesi, J;Sanders-Bush, E
通讯作者：
Sanders-Bush, E
DOI：
10.1016/s1096-4959(01)00525-5
发表时间：
2002-05-01
影响因子：
2.2
作者：
Cayre, M;Malaterre, J;Strambi, A
通讯作者：
Strambi, A