Serum lncRNAs in early pregnancy as potential biomarkers for the prediction of pregnancy-induced hypertension, including preeclampsia.
Serum lncRNAs in early pregnancy as potential biomarkers for the prediction of pregnancy-induced hypertension, including preeclampsia.
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妊娠早期血清lncRNA作为预测妊娠高血压（包括先兆子痫）的潜在生物标志物
DOI：
10.1016/j.omtn.2021.03.010
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发表时间：
2021-06-04
期刊：
影响因子：
--
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Dai C;Zhao C;Xu M;Sui X;Sun L;Liu Y;Su M;Wang H;Yuan Y;Zhang S;Shi J;Sun J;Li Y
长链非编码RNA（lncRNA）是生物调节的关键介质，作为疾病生物标志物具有诊断价值。我们探讨了妊娠早期血清lncRNA水平作为妊娠高血压（PIH），包括妊娠高血压（GH）和先兆子痫（PE）的潜在生物标志物。我们在妊娠20周前（临床诊断前）的孕妇中进行了一项两阶段巢式病例对照研究。筛选阶段使用人lncRNA微阵列评估了5对血清样本（5名PE患者和5名匹配对照）中的lncRNA表达谱。第二阶段通过定量实时聚合酶链反应（qRT-PCR）验证了通过随机游走法选择的8种候选lncRNA的水平。与匹配的正常血压妊娠（NP）妇女相比，患有PIH的妇女中8种lncRNA的血清水平显著增加（p < 0.001），与微阵列结果一致。此外，7个候选lncRNA与妊高征严重程度相关。Logistic回归分析显示，血清ENST 00000527727水平（比值比[OR]，1.113; 95%置信区间[CI]，1.024-1.209; p = 0.0113）和ENST 00000415029（OR，1.126; 95%CI，1.000-1.267; p = 0.0496）与不良妊娠结局相关，如胎儿生长受限（FGR）和妊高征胎盘植入。与候选lncRNA相关的9条通路已证实与妊高征相关。这项研究表明，在疾病的早期阶段，循环lncRNA的宏观预测和功能病理生理学相关性。此外，在妊娠早期更加重视lncRNA表达的检测可能会减少一些不利的母亲和胎儿的结果，如胎盘植入和FGR。
Long noncoding RNAs (lncRNAs) are key mediators of biological regulation with diagnostic value as disease biomarkers. We explored serum lncRNA levels in early pregnancy as potential biomarkers of pregnancy-induced hypertension (PIH), including gestational hypertension (GH) and preeclampsia (PE). We performed a two-phase nested case-control study in pregnant women before 20 weeks’ gestation (before clinical diagnosis). The screening phase assessed lncRNA expression profiles with a human lncRNA microarray in 5 pairs of serum samples (5 PE patients and 5 matched controls). The second phase validated levels of 8 candidate lncRNAs selected via the random walk method by quantitative real-time polymerase chain reaction (qRT-PCR). Serum levels of the 8 lncRNAs were markedly increased in women with PIH compared with matched normotensive pregnant (NP) women (p < 0.001), consistent with the microarray results. In addition, 7 candidate lncRNAs were correlated with PIH severity. Logistic regression analysis revealed that serum levels of ENST00000527727 (odds ratio [OR], 1.113; 95% confidence interval [CI], 1.024–1.209; p = 0.0113) and ENST00000415029 (OR, 1.126; 95% CI, 1.000–1.267; p = 0.0496) were associated with adverse pregnancy outcomes, such as fetal growth restriction (FGR) and placenta accreta of PIH. Nine pathways associated with the candidate lncRNAs had confirmed associations with PIH. This study indicates a macro predictive and functional pathophysiological correlation of circulating lncRNAs during the premature phase of the disease. Moreover, placing more emphasis on the detection of lncRNA expression in early pregnancy might reduce some adverse maternal and fetal outcomes, such as placenta accreta and FGR.
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影响因子：
5.6
作者：
Herraiz I;Simón E;Gómez-Arriaga PI;Martínez-Moratalla JM;García-Burguillo A;Jiménez EA;Galindo A
通讯作者：
Galindo A
影响因子：
14.9
作者：
Kuleshov MV;Jones MR;Rouillard AD;Fernandez NF;Duan Q;Wang Z;Koplev S;Jenkins SL;Jagodnik KM;Lachmann A;McDermott MG;Monteiro CD;Gundersen GW;Ma'ayan A
通讯作者：
Ma'ayan A
影响因子：
3.8
作者：
Irani, R. A.;Xia, Y.
通讯作者：
Xia, Y.
影响因子：
3.6
作者：
Kelly, Rachel S.;Croteau-Chonka, Damien C.;Lasky-Su, Jessica A.
通讯作者：
Lasky-Su, Jessica A.
DOI：
10.26355/eurrev_201806_15240
发表时间：
2018-06-01
影响因子：
3.3
作者：
Jiao, S.;Wang, S. -Y.;Huang, Y.
通讯作者：
Huang, Y.