Tunable plasmonic nanobubbles for cell theranostics.
Tunable plasmonic nanobubbles for cell theranostics.
复制标题
DOI：
10.1088/0957-4484/21/8/085102
复制
发表时间：
2010-02-26
期刊：
影响因子：
3.5
通讯作者：
Lapotko DO
中科院分区：
文献类型：
--
作者：
Lukianova-Hleb EY;Hanna EY;Hafner JH;Lapotko DO
将诊断和治疗过程结合为一体（治疗诊断学）并提高其对细胞水平的选择性可能会在各种研究和疾病系统中提供显着的益处，目前还没有有效的方法和药物支持。我们已经开发了一种新的方法的基础上的金纳米粒子产生的瞬态光热蒸汽纳米气泡，我们称之为等离子体纳米气泡（PNB）。在将金纳米颗粒（NP）递送和簇合到靶细胞之后，通过激光能量密度光学地产生和控制细胞内PNB。PNB作用在个体活细胞中从较低能量密度的非侵入性高灵敏度成像调整到较高能量密度的细胞膜破坏。我们已经实现了非侵入性的50倍放大的光学散射振幅与PNB（相对于纳米粒子），选择性的机械和快速损伤特定的细胞与更大的PNB，并通过损伤特定的信号的气泡的损伤的光学引导。因此，PNB在细胞水平上和在支持诊断、治疗和治疗指导的一个过程中充当可调治疗诊断剂。
Combining diagnostic and therapeutic processes into one (theranostics) and improving their selectivity to the cellular level may offer significant benefits in various research and disease systems and currently is not supported with efficient methods and agents. We have developed a novel method based on the gold nanoparticle-generated transient photothermal vapor nanobubbles, that we refer to as plasmonic nanobubbles (PNB). After delivery and clusterization of the gold nanoparticles (NP) to the target cells the intracellular PNBs were optically generated and controlled through the laser fluence. The PNB action was tuned in individual living cells from non-invasive high-sensitive imaging at lower fluence to disruption of the cellular membrane at higher fluence. We have achieved non-invasive 50-fold amplification of the optical scattering amplitude with the PNBs (relative to that of NPs), selective mechanical and fast damage to specific cells with bigger PNBs, and optical guidance of the damage through the damage-specific signals of the bubbles. Thus the PNBs acted as tunable theranostic agents at the cellular level and in one process that have supported diagnosis, therapy and guidance of the therapy.
登录
查看更多内容
DOI：
10.3181/0808-mr-250
发表时间：
2009-02
期刊：
Experimental biology and medicine (Maywood, N.J.)
影响因子：
--
作者：
Blanco E;Kessinger CW;Sumer BD;Gao J
通讯作者：
Gao J
影响因子：
2.4
作者：
Guzmán, HR;Nguyen, DX;Prausnitz, MR
通讯作者：
Prausnitz, MR
DOI：
10.1016/j.ijheatmasstransfer.2004.08.012
发表时间：
2005-01-01
影响因子：
5.2
作者：
Lapotko, D;Lukianova, E
通讯作者：
Lukianova, E
影响因子：
78.5
作者：
Kennedy, JE
通讯作者：
Kennedy, JE
影响因子：
5.5
作者：
Lapotko, Dmitri O.;Lukianova-Hleb, Ekaterina Y.;Oraevsky, Alexander A.
通讯作者：
Oraevsky, Alexander A.