Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal Strain Mucor circinelloides WJ11.
Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal Strain Mucor circinelloides WJ11.
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细胞质苹果酸酶在高产脂真菌环状毛霉WJ11产油能力中的作用
DOI：
10.3390/jof8030265
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发表时间：
2022-03-05
期刊：
影响因子：
--
通讯作者：
Song Y
中科院分区：
文献类型：
--
作者：
Fazili ABA;Shah AM;Naz T;Nosheen S;Yang W;Garre V;Majeed Y;Al-Sadoon MK;Song Y
环状毛霉是一种产油丝状真菌，由于其能够合成大量含有γ-亚麻酸（GLA）的脂质，对健康有重要益处，因此越来越受欢迎。苹果酸酶（ME）是一些真菌中NADPH的主要来源，已被发现可调节产油真菌的脂质积累。本研究评估了两个胞质ME基因cmalA和cmalB在M. circinelloides高脂产菌WJ11脂质积累中的作用。过表达cmalA和cmalB的菌株的代谢能活性分别提高了9.8倍和6.4倍，脂质含量分别提高了23.2%和5.8%，表明这些基因参与了脂质生物合成。由于脂质积累增加，cmalA和cmalB过表达菌株生物量中总GLA含量分别提高了11.42%和16.85%。我们的研究为探索cmalA基因作用的不同研究提供了重要的见解，而我们首次研究了cmalB基因在M. circinelloides WJ11菌株中的作用。
Mucor circinelloides, an oleaginous filamentous fungus, is gaining popularity due to its ability to synthesize significant amounts of lipids containing γ-linolenic acid (GLA) that have important health benefits. Malic enzyme (ME), which serves as the main source of NADPH in some fungi, has been found to regulate lipid accumulation in oleaginous fungi. In the present study, the role of two cytosolic ME genes, cmalA and cmalB, in the lipid accumulation of the M. circinelloides high-lipid-producing strain WJ11, was evaluated. Strains overexpressing cmalA and cmalB showed a 9.8- and 6.4-fold rise in specific ME activity, respectively, and an elevation of the lipid content by 23.2% and 5.8%, respectively, suggesting that these genes are involved in lipid biosynthesis. Due to increased lipid accumulation, overall GLA content in biomass was observed to be elevated by 11.42% and 16.85% in cmalA and cmalB overexpressing strains, respectively. Our study gives an important insight into different studies exploring the role of the cmalA gene, while we have for the first time investigated the role of the cmalB gene in the M. circinelloides WJ11 strain.
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影响因子：
5
作者：
Naz T;Yang J;Nosheen S;Sun C;Nazir Y;Mohamed H;Fazili ABA;Ullah S;Li S;Yang W;Garre V;Song Y
通讯作者：
Song Y
影响因子：
5.5
作者：
Nazir, Yusuf;Halim, Hafiy;Song, Yuanda
通讯作者：
Song, Yuanda
影响因子：
2.1
作者：
Li-Beisson, Yonghua;Peltier, Gilles
通讯作者：
Peltier, Gilles
DOI：
10.3390/jof7110934
发表时间：
2021-11-03
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Grubišić M;Mihajlovski K;Gruičić AM;Beluhan S;Šantek B;Ivančić Šantek M
通讯作者：
Ivančić Šantek M
DOI：
10.3390/jof7121061
发表时间：
2021-12-10
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Mohamed H;Naz T;Yang J;Shah AM;Nazir Y;Song Y
通讯作者：
Song Y