Strategies for Improvement of Lipid Production by Yeast Trichosporon oleaginosus from Lignocellulosic Biomass.
Strategies for Improvement of Lipid Production by Yeast Trichosporon oleaginosus from Lignocellulosic Biomass.
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DOI：
10.3390/jof7110934
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发表时间：
2021-11-03
期刊：
影响因子：
--
通讯作者：
Ivančić Šantek M
中科院分区：
文献类型：
--
作者：
Grubišić M;Mihajlovski K;Gruičić AM;Beluhan S;Šantek B;Ivančić Šantek M
微生物脂具有与植物油相似的脂肪酸组成，因此被认为是生产生物柴油的替代原料。含油性酵母在使用低成本的可再生原料如木质纤维生物质的过程中，会在细胞内积累相当数量的脂肪。本研究以碱处理玉米芯的水解液为原料，培养油酸毛孢霉。对不同的工艺配置进行了评价和比较，包括纤维素酶循环的单独水解发酵(SHF)和分批和补料分批的同时糖化发酵(SSF)。在低酶负荷条件下，添加2.5%(g−-1)底物的分批补料发酵的脂肪含量最高，为26.74g L−-1。对吸附在未水解木质纤维生物质上的纤维素酶进行了四轮间歇超滤处理。在不影响生产率和油脂产量的情况下，在第2-4轮中实现了30%的纤维素酶节省。在木质纤维浆料中添加吐温80提高了木质纤维原料中结构性碳水化合物的水解率。此外，在不影响酵母生长的情况下，添加吐温80可以提高脂肪产量和生产率。油酸酵母T.olaginosus是利用可再生木质纤维原料可持续高效生产油脂的优良菌株。
Microbial lipids have similar fatty acid composition to plant oils, and therefore, are considered as an alternative feedstock for biodiesel production. Oleaginous yeasts accumulate considerable amounts of lipids intracellularly during growth on low-cost renewable feedstocks such as lignocellulosic biomass. In this study, we cultivated yeast Trichosporon oleaginosus on hydrolysate of alkaline pretreated corn cobs. Different process configurations were evaluated and compared, including separate hydrolysis and fermentation (SHF) with cellulase recycle and simultaneous saccharification and fermentation (SSF) in batch and fed-batch mode. At low enzyme loading, the highest lipid concentration of 26.74 g L−1 was reached in fed-batch SSF fed with 2.5% (g g−1) substrate. Batch SHF was conducted for four rounds with recycling the cellulase adsorbed on unhydrolyzed lignocellulosic biomass. Thirty percent of cellulase saving was achieved for rounds 2–4 without compromising productivity and lipid yield. The addition of Tween 80 to lignocellulosic slurry improved the hydrolysis rate of structural carbohydrates in pretreated lignocellulosic biomass. Furthermore, supplementing the growth medium with Tween 80 improved lipid yield and productivity without affecting yeast growth. Oleaginous yeast T. oleaginosus is a promising strain for the sustainable and efficient production of lipids from renewable lignocellulosic feedstock.
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