Physical activity unveils the relationship between mitochondrial energetics, muscle quality, and physical function in older adults.
Physical activity unveils the relationship between mitochondrial energetics, muscle quality, and physical function in older adults.
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DOI：
10.1002/jcsm.12272
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Coen PM
中科院分区：
文献类型：
--
作者：
Distefano G;Standley RA;Zhang X;Carnero EA;Yi F;Cornnell HH;Coen PM
衰老肌肉中线粒体功能障碍的概念是非常有争议的。此外，新出现的证据表明，肌肉氧化能力和效率降低是老年人活动能力丧失的病因。在这里，我们假设在广泛的身体活动中研究表型良好的老年队列将揭示骨骼肌中的一系列线粒体功能，从而使我们能够更清楚地检查年龄本身对线粒体能量学的影响。这也使我们能够更清楚地定义线粒体能量学和肌肉脂质含量之间的关系，并对肌肉和身体功能进行临床相关评估。39名志愿者被招募到以下研究组：年轻活跃（YA，n = 2名女性/8名男性，年龄= 31.2 ± 5.4岁）、老年活跃（OA，n = 2名女性/8名男性，年龄= 67.5 ± 2.7岁）和老年久坐（OS，n = 8名女性/11名男性，年龄= 70.7 ± 4.7岁）。参与者完成了分级运动测试以确定健身（VO 2 peak），次极量运动测试以确定运动效率，并使用三轴臂带加速度计记录每日身体活动。线粒体能量学通过（i）31 P磁共振光谱和（ii）股外侧肌活检纤维束的呼吸测量来确定。股四头肌功能采用等速测力法，躯体功能采用短时间体能成套测验和爬楼梯试验。OS组每日体力活动能量消耗显著低于YA组和OA组。尽管健身是较高的YA相比，OA和OS，线粒体呼吸，最大线粒体容量，最大ATP产生/耗氧（P/O）的比率，和运动效率是相似的YA和OA组和OS显着降低。P/O比与运动效率。完成爬楼梯和重复椅子站立测试的时间对于OS显著更长。有趣的是，最大线粒体容量与肌肉收缩性能和身体功能相关。保持大量体力活动的老年人具有更好的线粒体能力，类似于高度活跃的年轻人，这与他们更好的肌肉质量、运动效率和身体表现有关。这表明线粒体可能是久坐老化相关病症的重要治疗靶点，所述病症包括肌肉减少症、运动障碍和身体功能丧失。
The concept of mitochondrial dysfunction in ageing muscle is highly controversial. In addition, emerging evidence suggests that reduced muscle oxidative capacity and efficiency underlie the aetiology of mobility loss in older adults. Here, we hypothesized that studying well‐phenotyped older cohorts across a wide range of physical activity would unveil a range of mitochondrial function in skeletal muscle and in turn allow us to more clearly examine the impact of age per se on mitochondrial energetics. This also enabled us to more clearly define the relationships between mitochondrial energetics and muscle lipid content with clinically relevant assessments of muscle and physical function. Thirty‐nine volunteers were recruited to the following study groups: young active (YA, n = 2 women/8 men, age = 31.2 ± 5.4 years), older active (OA, n = 2 women/8 men, age = 67.5 ± 2.7 years), and older sedentary (OS, n = 8 women/11 men, age = 70.7 ± 4.7 years). Participants completed a graded exercise test to determine fitness (VO2peak), a submaximal exercise test to determine exercise efficiency, and daily physical activity was recorded using a tri‐axial armband accelerometer. Mitochondrial energetics were determined by (i) 31P magnetic resonance spectroscopy and (ii) respirometry of fibre bundles from vastus lateralis biopsies. Quadriceps function was assessed by isokinetic dynamometry and physical function by the short physical performance battery and stair climb test. Daily physical activity energy expenditure was significantly lower in OS, compared with YA and OA groups. Despite fitness being higher in YA compared with OA and OS, mitochondrial respiration, maximum mitochondrial capacity, Maximal ATP production/Oxygen consumption (P/O) ratio, and exercise efficiency were similar in YA and OA groups and were significantly lower in OS. P/O ratio was correlated with exercise efficiency. Time to complete the stair climb and repeated chair stand tests were significantly greater for OS. Interestingly, maximum mitochondrial capacity was related to muscle contractile performance and physical function. Older adults who maintain a high amount of physical activity have better mitochondrial capacity, similar to highly active younger adults, and this is related to their better muscle quality, exercise efficiency, and physical performance. This suggests that mitochondria could be an important therapeutic target for sedentary ageing associated conditions including sarcopenia, dynapenia, and loss of physical function.
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影响因子：
3.3
作者：
Amati, Francesca;Dube, John J.;Goodpaster, Bret H.
通讯作者：
Goodpaster, Bret H.
影响因子：
15.9
作者：
Anderson, Ethan J.;Lustig, Mary E.;Neufer, P. Darrell
通讯作者：
Neufer, P. Darrell
DOI：
10.1111/j.1532-5415.1997.tb00986.x
发表时间：
1997-01-01
影响因子：
6.3
作者：
Fried, LP;Guralnik, JM
通讯作者：
Guralnik, JM
DOI：
10.1097/mco.0b013e328337819e
发表时间：
2010-05
影响因子：
3.1
作者：
Clark BC;Manini TM
通讯作者：
Manini TM
影响因子：
4.5
作者：
GOLLNICK, PD;SJODIN, B;SALTIN, B
通讯作者：
SALTIN, B