Induction of defense-related enzymes in patchouli inoculated with virulent Ralstonia solanacearum
Induction of defense-related enzymes in patchouli inoculated with virulent Ralstonia solanacearum
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接种强毒青枯菌的广藿香中防御相关酶的诱导
DOI：
10.1016/j.ejbt.2017.03.007
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发表时间：
2017-04
影响因子：
2.7
通讯作者：
Hong He
中科院分区：
文献类型：
--
作者：
Jian-Hui Xie;Ting-Ting Chai;Ran Xu;Dan Liu;Yu-Xiu Yang;Zhi-Cheng Deng;Hua Jin;Hong He
背景植物防御相关的抗氧化反应是植物抵抗病原菌入侵的重要防御机制，青枯菌是一种重要的植物病原菌。细菌性枯萎病（Bacterial wilt）由白腐病菌R.青枯病是最具破坏性的疾病，并导致广藿香，一种重要的芳香和药用植物，在东南亚的严重损失。本研究评价了百香果接种抗虫剂R后的防御反应。结果广藿香叶片和茎的基本酶活性存在差异，同一器官的上部和下部基本酶活性也存在差异。结果表明，接种病菌的广藿香叶片和茎中POD、SOD、PPO和PAL酶活性显著升高。与对照组相比，POD、PPO和PAL活性的变化幅度和速率均比SOD活性的变化幅度和速率大。青枯菌属PAGE同工酶分析表明，接种后的广藿香不仅防御相关酶活性升高，而且还诱导出了新的同工酶，其中POD同工酶有1条，SOD同工酶有2条，POD同工酶有2条，SOD同工酶有2条。青枯菌属
BackgroundDefense-related anti-oxidative response is a vital defense mechanism of plants against pathogen invasion.Ralstonia solanacearumis an important phytopathogen. Bacterial wilt caused byR. solanacearumis the most destructive disease and causes severe losses in patchouli, an important aromatic and medicinal plant in Southeast Asia. The present study evaluated the defense response of patchouli inoculated with virulentR. solanacearum.ResultsResults showed that the basic enzymatic activities differed not only between the leaves and stems but also between the upper and lower parts of the same organ of patchouli. POD, SOD, PPO, and PAL enzymatic activities were significantly elevated in leaves and stems from patchouli inoculated withR. solanacearumcompared to those in control. The variation magnitude and rate of POD, PPO, and PAL activities were more obvious than those of SOD in patchouli inoculated withR. solanacearum. PAGE isoenzymatic analysis showed that there were one new POD band and two new SOD bands elicited, and at least two isoformic POD bands and two SOD bands were observably intensified compared to the corresponding control.ConclusionOur results suggest that not only defense-related enzymatic activities were elevated but also the new isoenzymatic isoforms were induced in patchouli inoculated withR. solanacearum.
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影响因子：
3.7
作者：
Han Y;Li P;Gong S;Yang L;Wen L;Hou M
通讯作者：
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Jung, Woo-Jin;Mabood, Fazli;Smith, Donald L.
通讯作者：
Smith, Donald L.