Temporally coordinated expression of nuclear genes encoding chloroplast proteins in wheat promotes Puccinia striiformis f. sp. tritici infection.
Temporally coordinated expression of nuclear genes encoding chloroplast proteins in wheat promotes Puccinia striiformis f. sp. tritici infection.
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DOI：
10.1038/s42003-022-03780-4
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发表时间：
2022-08-22
影响因子：
5.9
通讯作者：
中科院分区：
文献类型：
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作者：
针对允许病原体茁壮成长的宿主过程在抗性育种中可能是非常宝贵的。在这里，我们生成了一个深度测序的转录组时间过程的条锈菌f。小麦上的感病性，并比较了来自三个小麦品种的数据集，这些小麦品种对两种测试的病原分离物具有不同的敏感性水平。我们寻找在易感宿主中特异性改变的基因作为可能支持殖民的候选基因。最显着的感染早期的Pst品种对之间的主机响应不同。然而，值得注意的是，核基因编码的叶绿体定位蛋白（NGCPs）表现出时间协调的表达谱不同，在以后的时间点在有关Pst的敏感性。破坏一个这样的NGCP，编码叶绿体定位的RNA结合蛋白TaCSP41a，导致较低的Pst易感性。因此，这些分析强调NGCP作为感染期间Pst操纵的主要目标，并指出TaCSP41a破坏作为育种计划的Pst抗性的潜在来源。条锈菌Puccinia striiformis f. triparum（Pst）感染揭示了编码叶绿体定位蛋白的核基因在感染期间被操纵，并突出了TaCSP41a破坏作为抗性育种的目标。
Targeting host processes that allow pathogens to thrive can be invaluable in resistance breeding. Here, we generated a deep-sequencing transcriptome time course for Puccinia striiformis f. sp. tritici (Pst) infection on wheat and compared datasets from three wheat varieties with different levels of susceptibility to two tested pathogen isolates. We sought genes specifically altered in a susceptible host as candidates that might support colonisation. Host responses differed between Pst-varietal pairs most prominently early during infection. Notably, however, nuclear genes encoding chloroplast-localised proteins (NGCPs) exhibited temporal coordination of expression profiles that differed at later time points in relation to Pst susceptibility. Disrupting one such NGCP, encoding the chloroplast-localised RNA binding protein TaCSP41a, led to lower Pst susceptibility. These analyses thus highlight NGCPs as prime targets for Pst manipulation during infection and point to TaCSP41a disruption as a potential source of Pst resistance for breeding programmes. A transcriptome time course of Puccinia striiformis f. sp. tritici (Pst) infection reveals nuclear genes encoding chloroplast-localized proteins are manipulated during infection and highlights TaCSP41a disruption as a target for resistance breeding.
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影响因子：
4.4
作者：
Dobon A;Bunting DC;Cabrera-Quio LE;Uauy C;Saunders DG
通讯作者：
Saunders DG
影响因子：
4.9
作者：
Coram, Tristan E.;Wang, Meinan;Chen, Xianming
通讯作者：
Chen, Xianming
影响因子：
2.7
作者：
Chang, Qing;Liu, Jie;Kang, Zhensheng
通讯作者：
Kang, Zhensheng
DOI：
10.1093/plcell/koab049
发表时间：
2021-07-02
期刊：
The Plant cell
影响因子：
--
作者：
Corredor-Moreno P;Minter F;Davey PE;Wegel E;Kular B;Brett P;Lewis CM;Morgan YML;Macías Pérez LA;Korolev AV;Hill L;Saunders DGO
通讯作者：
Saunders DGO
影响因子：
46.9
作者：
Bray, Nicolas L.;Pimentel, Harold;Pachter, Lior
通讯作者：
Pachter, Lior