The branched-chain amino acid aminotransferase TaBCAT1 modulates amino acid metabolism and positively regulates wheat rust susceptibility.
The branched-chain amino acid aminotransferase TaBCAT1 modulates amino acid metabolism and positively regulates wheat rust susceptibility.
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DOI：
10.1093/plcell/koab049
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发表时间：
2021-07-02
期刊：
影响因子：
--
通讯作者：
Saunders DGO
中科院分区：
文献类型：
--
作者：
Corredor-Moreno P;Minter F;Davey PE;Wegel E;Kular B;Brett P;Lewis CM;Morgan YML;Macías Pérez LA;Korolev AV;Hill L;Saunders DGO
支链氨基酸氨基转移酶是小麦黄锈病和秆锈病发展所必需的，这两种病害是世界范围内对小麦经济破坏最大的两种病害。植物病原体抑制防御反应以逃避识别并促进成功定殖。虽然确定病原体入侵所必需的基因传统上依赖于筛选突变群体，后基因组时代提供了一个机会，开发新的方法，加速识别。在这里，对68个病原体感染的面包小麦（Triticum aestivum）品种（包括三个具有可变易感性水平的品种（奥克利、Solstice和圣地亚哥））的RNA-seq分析揭示了支链氨基酸氨基转移酶（称为TaBCAT 1）作为小麦锈病易感性的正调节因子。我们表明，TaBCAT 1是所需的黄色和茎锈病感染和支链氨基酸（BCAA）代谢可能的功能，因为TaBCAT 1破坏突变体的BCAA水平升高。TaBCAT 1突变体还表现出水杨酸（SA）水平的增加和相关防御基因的表达增强，表明BCAA调节，通过TaBCAT 1，在SA依赖的防御激活中具有关键作用。我们还确定了支链氨基酸水平和小麦抗条锈病感染之间的关联。这些发现提供了对SA介导的小麦防御反应的深入了解，并突出了BCAA代谢在防御反应中的作用。此外，TaBCAT 1可以被操纵，以潜在地提供对全球小麦的两种最具经济破坏性的疾病的抗性。
A branched-chain amino acid aminotransferase is essential for progression of wheat yellow and stem rust, two of the most economically damaging diseases of wheat worldwide. Plant pathogens suppress defense responses to evade recognition and promote successful colonization. Although identifying the genes essential for pathogen ingress has traditionally relied on screening mutant populations, the post-genomic era provides an opportunity to develop novel approaches that accelerate identification. Here, RNA-seq analysis of 68 pathogen-infected bread wheat (Triticum aestivum) varieties, including three (Oakley, Solstice and Santiago) with variable levels of susceptibility, uncovered a branched-chain amino acid aminotransferase (termed TaBCAT1) as a positive regulator of wheat rust susceptibility. We show that TaBCAT1 is required for yellow and stem rust infection and likely functions in branched-chain amino acid (BCAA) metabolism, as TaBCAT1 disruption mutants had elevated BCAA levels. TaBCAT1 mutants also exhibited increased levels of salicylic acid (SA) and enhanced expression of associated defense genes, indicating that BCAA regulation, via TaBCAT1, has a key role in SA-dependent defense activation. We also identified an association between the levels of BCAAs and resistance to yellow rust infection in wheat. These findings provide insight into SA-mediated defense responses in wheat and highlight the role of BCAA metabolism in the defense response. Furthermore, TaBCAT1 could be manipulated to potentially provide resistance to two of the most economically damaging diseases of wheat worldwide.
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影响因子：
3.3
作者：
Bueno-Sancho V;Persoons A;Hubbard A;Cabrera-Quio LE;Lewis CM;Corredor-Moreno P;Bunting DCE;Ali S;Chng S;Hodson DP;Madariaga Burrows R;Bryson R;Thomas J;Holdgate S;Saunders DGO
通讯作者：
Saunders DGO
影响因子：
64.5
作者：
Hartmann, Michael;Zeier, Tatyana;Zeier, Juergen
通讯作者：
Zeier, Juergen
树突镜：大型系统发育树的互动观众。
影响因子：
3
作者：
Huson, Daniel H.;Richter, Daniel C.;Rausch, Christian;Dezulian, Tobias;Franz, Markus;Rupp, Regula
通讯作者：
Rupp, Regula
影响因子：
56.9
作者：
Fan, Jun;Crooks, Casey;Lamb, Chris
通讯作者：
Lamb, Chris
影响因子：
1.5
作者：
Jayaraj, J;Muthukrishnan, S;Velazhahan, R
通讯作者：
Velazhahan, R