Dietary Glutamine Inclusion Regulates Immune and Antioxidant System, as Well as Programmed Cell Death in Fish to Protect against Flavobacterium columnare Infection.
Dietary Glutamine Inclusion Regulates Immune and Antioxidant System, as Well as Programmed Cell Death in Fish to Protect against Flavobacterium columnare Infection.
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膳食中添加谷氨酰胺可调节鱼类的免疫和抗氧化系统以及程序性细胞死亡，以防止柱状黄杆菌感染
DOI：
10.3390/antiox11010044
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发表时间：
2021-12-26
期刊：
影响因子：
--
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Jiao C;Zou J;Chen Z;Zheng F;Xu Z;Lin YH;Wang Q
动物对病原体感染的易感性受营养状况的显著影响。本研究以黄颡鱼（Pelteobagrus fulvidraco）为模型，验证了谷氨酰胺在细菌感染过程中的保护作用主要与其对免疫和抗氧化系统、细胞凋亡和自噬的调节有关。饲料中添加谷氨酰胺显著提高了鱼的生长性能和饲料利用率。在柱状黄杆菌攻击后，补充谷氨酰胺可通过NF-κB信号促进头肾和脾脏中il-8和il-1β的表达，但抑制肠道和鳃中的过度炎症。此外，饮食中加入谷氨酰胺也增强了系统的抗氧化能力。组织学分析表明谷氨酰胺对鳃结构具有保护作用。进一步的研究表明，谷氨酰胺可减轻细菌感染引起的细胞凋亡，同时沿着caspase-3蛋白水平的降低和凋亡相关基因表达的减少。此外，谷氨酰胺还显示出在自噬中的抑制作用，这是由于mTOR信号通路的激活增加。因此，我们的研究首次阐明了谷氨酰胺在鱼类免疫和抗氧化系统中的调节作用，并报道了其在细菌感染过程中对鱼类细胞凋亡和自噬的抑制作用。
The susceptibility of animals to pathogenic infection is significantly affected by nutritional status. The present study took yellow catfish (Pelteobagrus fulvidraco) as a model to test the hypothesis that the protective roles of glutamine during bacterial infection are largely related to its regulation on the immune and antioxidant system, apoptosis and autophagy. Dietary glutamine supplementation significantly improved fish growth performance and feed utilization. After a challenge with Flavobacterium columnare, glutamine supplementation promoted il-8 and il-1β expression via NF-κB signaling in the head kidney and spleen, but inhibited the over-inflammation in the gut and gills. Additionally, dietary glutamine inclusion also enhanced the systematic antioxidant capacity. Histological analysis showed the protective role of glutamine in gill structures. Further study indicated that glutamine alleviated apoptosis during bacterial infection, along with the reduced protein levels of caspase-3 and the reduced expression of apoptosis-related genes. Moreover, glutamine also showed an inhibitory role in autophagy which was due to the increased activation of the mTOR signaling pathway. Thus, our study for the first time illustrated the regulatory roles of glutamine in the fish immune and antioxidant system, and reported its inhibitory effects on fish apoptosis and autophagy during bacterial infection.
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影响因子：
9.2
作者：
Gong G;Dan C;Xiao S;Guo W;Huang P;Xiong Y;Wu J;He Y;Zhang J;Li X;Chen N;Gui JF;Mei J
通讯作者：
Mei J
影响因子：
7.5
作者：
Deretic V
通讯作者：
Deretic V
鱼类的粘膜免疫系统：耐受共生体同时对抗病原体的进化。
影响因子：
4.7
作者：
Gomez, Daniela;Sunyer, J. Oriol;Salinas, Irene
通讯作者：
Salinas, Irene
DOI：
10.1016/j.cbpa.2015.09.012
发表时间：
2016-01-01
影响因子：
2.3
作者：
Coutinho, F.;Castro, C.;Peres, H.
通讯作者：
Peres, H.
影响因子：
2.7
作者：
Brouwer IJ;Out-Luiting JJ;Vermeer MH;Tensen CP
通讯作者：
Tensen CP