Photodynamic effects of methylene blue-loaded polymeric nanoparticles on dental plaque bacteria.
Photodynamic effects of methylene blue-loaded polymeric nanoparticles on dental plaque bacteria.
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DOI：
10.1002/lsm.21069
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发表时间：
2011-09
影响因子：
2.4
通讯作者：
Soukos, Nikolaos S.
中科院分区：
文献类型：
--
作者：
Klepac-Ceraj, Vanja;Patel, Niraj;Song, Xiaoqing;Holewa, Colleen;Patel, Chitrang;Kent, Ralph;Amiji, Mansoor M.;Soukos, Nikolaos S.
光动力疗法（PDT）越来越多地被用于治疗口腔感染。在此，我们利用带正电荷或负电荷的亚甲基蓝（MB）负载聚乳酸-羟基乙酸（PLGA）纳米颗粒和665 nm的红光，在体外研究PDT对人牙菌斑细菌的影响。对14例慢性牙周炎患者进行龈下菌斑采集。将7名患者的菌斑微生物悬浮液用阴离子、阳离子PLGA纳米颗粒（50 μg/ml相当于MB）或游离MB （50 μg/ml）致敏20分钟，然后在功率密度为100 mW/cm2的红光下照射5分钟。从7名患者获得的牙菌斑接种物在96孔板的血琼脂上培养多微生物口腔生物膜，同样暴露于PDT。处理后，通过计算菌落形成单位的数量来计算生存分数。与负离子负载MB纳米粒子和游离MB相比，阳离子负载MB纳米粒子在浮游和生物膜阶段都表现出更大的细菌光毒性，但结果没有显著差异（p>0.05）。阳离子负载MB的PLGA纳米颗粒具有作为PDT系统中MB载体的潜力。
Photodynamic therapy (PDT) is increasingly being explored for treatment of oral infections. Here, we investigate the effect of PDT on human dental plaque bacteria in vitro using methylene blue (MB)-loaded poly(lactic-co-glycolic) (PLGA) nanoparticles with a positive or negative charge and red light at 665 nm. Subgingival plaque samples were obtained from 14 patients with chronic periodontitis. Suspensions of plaque microorganisms from seven patients were sensitized with anionic, cationic PLGA nanoparticles (50 μg/ml equivalent to MB) or free MB (50 μg/ml) for 20 min followed by exposure to red light for 5 min with a power density of 100 mW/cm2. Polymicrobial oral biofilms, which were developed on blood agar in 96-well plates from dental plaque inocula obtained from seven patients, were also exposed to PDT as above. Following the treatment, survival fractions were calculated by counting the number of colony-forming units. The cationic MB-loaded nanoparticles exhibited greater bacterial phototoxicity in both planktonic and biofilm phase compared to anionic MB-loaded nanoparticles and free MB, but results were not significantly different (p>0.05). Cationic MB-loaded PLGA nanoparticles have the potential to be used as carriers of MB for PDT systems.
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影响因子：
--
作者：
Kumar, MNVR;Mohapatra, SS;Lehr, CM
通讯作者：
Lehr, CM
影响因子：
--
作者：
Sedlacek, M. J.;Walker, C.
通讯作者：
Walker, C.
影响因子：
6.7
作者：
del Pozo, J. L.;Patel, R.
通讯作者：
Patel, R.
影响因子：
3.5
作者：
Fontana CR;Abernethy AD;Som S;Ruggiero K;Doucette S;Marcantonio RC;Boussios CI;Kent R;Goodson JM;Tanner AC;Soukos NS
通讯作者：
Soukos NS
影响因子：
5.2
作者：
BROWN, MRW;ALLISON, DG;GILBERT, P
通讯作者：
GILBERT, P