Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH(2)-A Novel Magnetic Nanotube-Polyethylene Glycol Amine Composite-And Its Applications for Biodiesel Production.
Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH(2)-A Novel Magnetic Nanotube-Polyethylene Glycol Amine Composite-And Its Applications for Biodiesel Production.
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DOI：
10.3390/ijms222111956
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发表时间：
2021-11-04
影响因子：
5.6
通讯作者：
Yan Y
中科院分区：
文献类型：
--
作者：
Abdulmalek SA;Li K;Wang J;Ghide MK;Yan Y
本文描述了一种新型的超顺磁性多壁纳米管与四臂聚乙二醇胺聚合物的纳米复合材料的成功合成（mMWCNTs@4-arm-PEG-NH2）。然后在适当的条件下，将该复合材料作为米根霉和念珠菌脂肪酶共价共固定化的载体。共固定化脂肪酶（CIL-mMWCNTs@4-arm-PEG-NH2）的最大比活性为99.626U/mg蛋白，是游离ROL的34.5倍，热稳定性得到了很大提高。最显著的是，利用CIL-mMWCNTs@4-arm-PEG-NH2在超声条件下从废食用油中制备生物柴油，在120 min内，生物柴油的转化率达到97.64%。这是由于ROL和CRL之间的协同效应以及超声波辅助酶促过程，在短反应时间内提高了生物柴油的产量。此外，在重复使用10次后，共固定化脂肪酶仍保持78.55%以上的生物柴油产率，表现出良好的操作稳定性，具有实际应用价值。因此，将新型载体、具有协同效应的共固定化脂肪酶与超声辅助酶促反应相结合，在生物柴油生产和各种工业应用中具有潜在的应用前景。
This article describes the successful synthesis of a novel nanocomposite of superparamagnetic multi-walled nanotubes with a four-arm polyethylene glycol amine polymer (mMWCNTs@4-arm-PEG-NH2). This composite was then employed as a support for the covalent co-immobilization of Rhizopus oryzae and Candida rugosa lipases under appropriate conditions. The co-immobilized lipases (CIL-mMWCNTs@4-arm-PEG-NH2) exhibited maximum specific activity of 99.626U/mg protein, which was 34.5-fold superior to that of free ROL, and its thermal stability was greatly improved. Most significantly, CIL-mMWCNTs@4-arm-PEG-NH2 was used to prepare biodiesel from waste cooking oil under ultrasound conditions, and within 120 min, the biodiesel conversion rate reached 97.64%. This was due to the synergy effect between ROL and CRL and the ultrasound-assisted enzymatic process, resulting in an increased biodiesel yield in a short reaction time. Moreover, after ten reuse cycles, the co-immobilized lipases still retained a biodiesel yield of over 78.55%, exhibiting excellent operational stability that is attractive for practical applications. Consequently, the combined use of a novel designed carrier, the co-immobilized lipases with synergy effect, and the ultrasound-assisted enzymatic reaction exhibited potential prospects for future applications in biodiesel production and various industrial applications.
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影响因子：
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作者：
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影响因子：
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