Genome-Wide Analysis and Function Prediction of Long Noncoding RNAs in Sheep Pituitary Gland Associated with Sexual Maturation
Genome-Wide Analysis and Function Prediction of Long Noncoding RNAs in Sheep Pituitary Gland Associated with Sexual Maturation
复制标题
绵羊垂体中与性成熟相关的长非编码 RNA 的全基因组分析和功能预测
DOI：
10.3390/genes11030320
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发表时间：
2020-03
期刊：
影响因子：
3.5
通讯作者：
Zhang Yanli
中科院分区：
文献类型：
--
作者：
Yang Hua;Ma Jianyu;Wang Zhibo;Yao Xiaolei;Zhao Jie;Zhao Xinyue;Wang Feng;Zhang Yanli
长链非编码RNA（lncRNA）在绵羊生殖系统的下丘脑-垂体-睾丸（HPT）轴中起着重要作用。垂体在HPT轴中起连接作用。然而，很少有人知道他们的表达模式和潜在的作用，在垂体。为了探索调控雄性绵羊垂体发育和性成熟的潜在lncRNA，我们构建了未成熟和成熟绵羊垂体cDNA文库（分别为3月龄TM和9月龄NM，n = 3），并进行lncRNA和mRNA高通量测序。首先，比较分析lncRNA和mRNA的表达。共鉴定出2417个已知的lncRNA和1256个新的lncRNA。在两组垂体中共检测到193条差异表达的lncRNAs和1407条差异表达的mRNA。根据lncRNA的靶基因预测和功能富集分析，构建了mRNA-lncRNA相互作用网络。进一步筛选了五种富集生长和生殖相关途径的候选lncRNA及其靶基因HPD 17 B12、DCBLD 2、PDPK 1、GPX 3和DLL 1。最后，在体外绵羊垂体细胞中验证了候选lncRNA TCONS_00066406与其靶基因HSD 17 B12的相互作用。本研究系统地研究了雄性绵羊垂体中lncRNA及其mRNA的表达，揭示了lncRNA在雄性生殖中的潜在作用。
Long noncoding RNA (lncRNA) plays a crucial role in the hypothalamic-pituitary-testis (HPT) axis associated with sheep reproduction. The pituitary plays a connecting role in the HPT axis. However, little is known of their expression pattern and potential roles in the pituitary gland. To explore the potential lncRNAs that regulate the male sheep pituitary development and sexual maturation, we constructed immature and mature sheep pituitary cDNA libraries (three-month-old, TM, and nine-month-old, NM, respectively, n = 3) for lncRNA and mRNA high-throughput sequencing. Firstly, the expression of lncRNA and mRNA were comparatively analyzed. 2417 known lncRNAs and 1256 new lncRNAs were identified. Then, 193 differentially expressed (DE) lncRNAs and 1407 DE mRNAs were found in the pituitary between the two groups. Moreover, mRNA-lncRNA interaction network was constructed according to the target gene prediction of lncRNA and functional enrichment analysis. Five candidate lncRNAs and their targeted genes HSD17B12, DCBLD2, PDPK1, GPX3 and DLL1 that enriched in growth and reproduction related pathways were further filtered. Lastly, the interaction of candidate lncRNA TCONS_00066406 and its targeted gene HSD17B12 were validated in in vitro of sheep pituitary cells. Our study provided a systematic presentation of lncRNAs and mRNAs in male sheep pituitary, which revealed the potential role of lncRNA in male reproduction.
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影响因子：
2.9
作者：
Chen, Shan-Wen;Zhu, Jing;Wang, Peng-Yuan
通讯作者：
Wang, Peng-Yuan
DOI：
10.1016/j.bbrc.2015.02.100
发表时间：
2015-03
影响因子：
3.1
作者：
Naoko Tsukamoto-Yamauchi;T. Terasaka;Y. Iwasaki;F. Otsuka
通讯作者：
Naoko Tsukamoto-Yamauchi;T. Terasaka;Y. Iwasaki;F. Otsuka
影响因子：
14.9
作者：
Volders PJ;Verheggen K;Menschaert G;Vandepoele K;Martens L;Vandesompele J;Mestdagh P
通讯作者：
Mestdagh P
未成熟和成熟大鼠垂体前叶中长非编码RNA的鉴定
影响因子：
4.6
作者：
Han DX;Sun XL;Fu Y;Wang CJ;Liu JB;Jiang H;Gao Y;Chen CZ;Yuan B;Zhang JB
通讯作者：
Zhang JB
microRNA-21-3p和microRNA-433差异表达在大鼠垂体前叶细胞FSH调节中的作用
影响因子：
--
作者：
Han DX;Sun XL;Xu MQ;Chen CZ;Jiang H;Gao Y;Yuan B;Zhang JB
通讯作者：
Zhang JB