Simultaneous monitoring of the drug release and antitumor effect of a novel drug delivery system-MWCNTs/DOX/TC.
Simultaneous monitoring of the drug release and antitumor effect of a novel drug delivery system-MWCNTs/DOX/TC.
复制标题
同时监测新型药物递送系统-MWCNTs/DOX/TC的药物释放和抗肿瘤作用
DOI：
10.1080/10717544.2016.1233592
复制
发表时间：
2017-11
期刊：
影响因子：
6
通讯作者：
Leng X
中科院分区：
文献类型：
--
作者：
Dong X;Sun Z;Wang X;Zhu D;Liu L;Leng X
摘要药物释放和疗效监测是药物释放系统开发的关键。我们的初步研究表明，与原始多壁碳纳米管（MWCNTs）相比，转录反式激活因子（达特）-壳聚糖功能化多壁碳纳米管（MWCNTs-TC）是癌症治疗中更有希望的药物输送候选物。在本研究中，多壁碳纳米管/TC为基础的药物输送系统开发的抗癌药物，阿霉素（DOX）。评价了载药量、体外释放曲线、细胞摄取和细胞毒性。更重要的是，通过无创荧光和生物发光成像评价了MWCNTs/DOX/TC的体内药物释放和抗肿瘤作用。结果表明，MWCNTs/DOX/TC可被BEL-7402肝癌细胞有效摄取。MWCNTs/DOX/TC在较低pH条件下释放较快，有利于药物的胞内释放。通过无创性荧光成像和发光成像技术同时监测DOX在体内的释放过程和动物模型的抗肿瘤作用，显示了MWCNTs/DOX/TC在肿瘤治疗中的应用潜力。
Abstract Monitoring drug release and therapeutic efficacy is crucial for developing drug delivery systems. Our preliminary study demonstrated that, as compared with pristine multiwalled carbon nanotubes (MWCNTs), transactivator of transcription (TAT)-chitosan functionalized MWCNTs (MWCNTs-TC) were a more promising candidate for drug delivery in cancer therapy. In the present study, a MWCNTs/TC-based drug delivery system was developed for an anticancer drug, doxorubicin (DOX). The drug loading and in vitro release profiles, cellular uptake and cytotoxicity were assessed. More importantly, the in vivo drug release and antitumor effect of MWCNTs/DOX/TC were evaluated by noninvasive fluorescence and bioluminescence imaging. It was demonstrated that MWCNTs/DOX/TC can be efficiently taken up by BEL-7402 hepatoma cells. The release of DOX from MWCNTs/DOX/TC was faster under lower pH condition, which was beneficial for intrcellular drug release. The in vivo release process of DOX and antitumor effect in animal model were monitored simultaneously by noninvasive fluorescence and luminescence imaging, which demonstrated the application potential of MWCNTs/DOX/TC for cancer therapy.
登录
查看更多内容
影响因子：
10.8
作者：
Mu Q;Broughton DL;Yan B
通讯作者：
Yan B
影响因子：
4.9
作者：
Das, Basanta K.;Tlili, Chaker;Mulchandani, Ashok
通讯作者：
Mulchandani, Ashok
影响因子：
6
作者：
Li, Haixia;Zhang, Nan;Zhang, Zhenzhong
通讯作者：
Zhang, Zhenzhong
影响因子：
9.7
作者：
Misra, R. D. K.;Depan, D.;Shah, J. S.
通讯作者：
Shah, J. S.
影响因子：
10.8
作者：
Duch MC;Budinger GR;Liang YT;Soberanes S;Urich D;Chiarella SE;Campochiaro LA;Gonzalez A;Chandel NS;Hersam MC;Mutlu GM
通讯作者：
Mutlu GM