Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021.
Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021.
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DOI：
10.3390/tropicalmed7120420
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发表时间：
2022-12-07
影响因子：
2.9
通讯作者：
Faye O
中科院分区：
文献类型：
--
作者：
Dieng I;Barry MA;Talla C;Sow B;Faye O;Diagne MM;Sene O;Ndiaye O;Diop B;Diagne CT;Fall G;Sall AA;Loucoubar C;Faye O
塞内加尔是登革热高发区。自2017年以来，全国许多地区每年都出现疫情，其标志是DENV 1 -3的共同传播。2021年10月8日，位于该国北部的罗索卫生站（综合征监测网络的哨点）发生登革热病毒疫情，并通报给达喀尔巴斯德研究所的世卫组织虫媒病毒和出血热病毒合作中心。随后派出一个多学科小组进行流行病学和病毒学调查。本研究描述了塞内加尔暴发期间使用快速诊断试验（RDT）联合检测登革热病毒非结构蛋白1（NS 1）和IgM/IgG的调查结果。为了确认，还在达喀尔的参考实验室通过实时RT-PCR和IgM ELISA检测了样本。使用纳米孔技术对qRT-PCR阳性样品进行全基因组测序。病毒学分析在173名入组患者中获得了102例阳性病例（RT-PCR，NS 1抗原检测和/或IgM）;有趣的是，病毒血清分型显示，此次疫情是由DENV-1引起的，这是一种与三年前罗索疫情期间涉及的DENV-2不同的血清型，表明血清型更换。几乎所有现场检测的NS 1阳性样品均通过qRT-PCR确认，一致性为92.3%。菌株的全基因组测序和系统发育分析表明，罗索重新引入了一种DENV-1菌株，该菌株与五年前在Louga地区爆发的菌株不同。研究结果呼吁塞内加尔加强登革热病毒监测，广泛部署DENV抗原检测，以便现场诊断疑似病例和早期发现疫情。这项工作强调了持续监测流行血清型的必要性，这对于更好地了解全国各地的病毒流行病学至关重要。
Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country.
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影响因子：
3.7
作者：
Dieng I;Ndione MHD;Fall C;Diagne MM;Diop M;Gaye A;Barry MA;Diop B;Ndiaye M;Bousso A;Fall G;Loucoubar C;Faye O;Sall AA;Faye O
通讯作者：
Faye O
DOI：
10.3390/v13010057
发表时间：
2021-01-04
期刊：
Viruses
影响因子：
--
作者：
Dieng I;Cunha M;Diagne MM;Sembène PM;Zanotto PMA;Faye O;Faye O;Sall AA
通讯作者：
Sall AA
影响因子：
4.6
作者：
Gaye A;Ndiaye T;Sy M;Deme AB;Thiaw AB;Sene A;Ndiaye C;Diedhiou Y;Mbaye AM;Ndiaye I;Tomkins-Tinch C;Gomis JF;Badiane AS;MacInnis B;Park DJ;Ndiaye M;Sy N;Sabeti PC;Siddle KJ;Ndiaye D
通讯作者：
Ndiaye D
DOI：
10.1126/science.aac5017
发表时间：
2015-09-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Katzelnick LC;Fonville JM;Gromowski GD;Bustos Arriaga J;Green A;James SL;Lau L;Montoya M;Wang C;VanBlargan LA;Russell CA;Thu HM;Pierson TC;Buchy P;Aaskov JG;Muñoz-Jordán JL;Vasilakis N;Gibbons RV;Tesh RB;Osterhaus AD;Fouchier RA;Durbin A;Simmons CP;Holmes EC;Harris E;Whitehead SS;Smith DJ
通讯作者：
Smith DJ
影响因子：
2.2
作者：
Dieng I;Diarra M;Diagne MM;Faye M;Dior Ndione MH;Ba Y;Diop M;Ndiaye EH;Marinho de Andrade Zanotto P;Diop B;Ndiaye M;Bousso A;Dia N;Diallo M;Barry A;Fall G;Loucoubar C;Sall AA;Faye O;Faye O
通讯作者：
Faye O