Elevated IL-17 levels in semi-immune anaemic mice infected with Plasmodium berghei ANKA.
Elevated IL-17 levels in semi-immune anaemic mice infected with Plasmodium berghei ANKA.
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DOI：
10.1186/s12936-018-2257-x
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发表时间：
2018-04-17
期刊：
影响因子：
3
通讯作者：
Hirayama K
中科院分区：
文献类型：
--
作者：
Helegbe GK;Huy NT;Yanagi T;Shuaibu MN;Kikuchi M;Cherif MS;Hirayama K
宿主的炎症细胞因子和遗传背景的改变有助于疟疾感染的结果。尽管IL-17在感染中具有良好的保护作用，但很少有人进一步了解其在慢性/地方性严重疟疾贫血发病机制中的重要性。因此，本研究的目的是评估贫血条件下IL-17水平及其与宿主遗传因素的关系。将2株小鼠（Balb/c和CBA）杂交得到F1后代，（F1, Balb/c， CBA）经6个周期的伯氏疟原虫（ANKA株）感染和氯喹/乙胺处理产生半免疫状态。细胞因子水平和抗体产生动力学、CD4+CD25+T调节性细胞分别通过珠细胞复合检测试剂盒、ELISA和FACs检测。Balb/c和F1在明显低寄生血症下观察到高存活率和高Hb损失。此外，Balb/c， F1的IgG水平比CBA高2倍。CBA组CD4+CD25+ Treg细胞较低；Balb/c、F1中IL-4、IFN-γ、IL-12α、IL-17显著升高（p < 0.05）。综上所述，IL-17水平升高与IL-4、IL-12α和IFN-γ水平升高可能是一种保护标志，其机制可能受宿主因素控制。进一步研究F1和Balb/c之间的F2将有助于评估这些基因是分离还是进一步分离。本文的在线版本（10.1186/s12936-018-2257-x）包含补充材料，授权用户可以使用。
Alterations in inflammatory cytokines and genetic background of the host contribute to the outcome of malaria infection. Despite the promising protective role of IL-17 in infections, little attention is given to further understand its importance in the pathogenesis of severe malaria anaemia in chronic/endemic situations. The objective of this study, therefore, was to evaluate IL-17 levels in anaemic condition and its association with host genetic factors. Two mice strains (Balb/c and CBA) were crossed to get the F1 progeny, and were (F1, Balb/c, CBA) taken through 6 cycles of Plasmodium berghei (ANKA strain) infection and chloroquine/pyrimethamine treatment to generate semi-immune status. Cytokine levels and kinetics of antibody production, CD4+CD25+T regulatory cells were evaluated by bead-based multiplex assay kit, ELISA and FACs, respectively. High survival with high Hb loss at significantly low parasitaemia was observed in Balb/c and F1. Furthermore, IgG levels were two times higher in Balb/c, F1 than CBA. While CD4+CD25+ Treg cells were lower in CBA; IL-4, IFN-γ, IL-12α and IL-17 were significantly higher (p < 0.05) in Balb/c, F1. In conclusion, elevated IL-17 levels together with high IL-4, IL-12α and IFN-γ levels may be a marker of protection, and the mechanism may be controlled by host factor (s). Further studies of F2 between the F1 and Balb/c will be informative in evaluating if these genes are segregated or further apart. The online version of this article (10.1186/s12936-018-2257-x) contains supplementary material, which is available to authorized users.
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影响因子：
5.4
作者：
Cooper, Andrea M.
通讯作者：
Cooper, Andrea M.
影响因子：
2
作者：
Mirghani, Hiba A.;Eltahir, Hatim G.;Adam, Ishag
通讯作者：
Adam, Ishag
影响因子：
4.4
作者：
Abrams, ET;Brown, H;Rogerson, SJ
通讯作者：
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慢性暴露后，红细胞破坏的速率在感染了伯氏疟原虫的不同小鼠的不同菌株中各不相同。
影响因子：
3
作者：
Helegbe GK;Huy NT;Yanagi T;Shuaibu MN;Yamazaki A;Kikuchi M;Yasunami M;Hirayama K
通讯作者：
Hirayama K
影响因子：
6.4
作者：
Huang, WT;Na, L;Schwarzenberger, P
通讯作者：
Schwarzenberger, P