Vertical and horizontal corneal epithelial thickness profile using ultra-high resolution and long scan depth optical coherence tomography.
Vertical and horizontal corneal epithelial thickness profile using ultra-high resolution and long scan depth optical coherence tomography.
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DOI：
10.1371/journal.pone.0097962
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Wu S;Tao A;Jiang H;Xu Z;Perez V;Wang J
使用超长扫描深度和超高分辨率谱域光学相干断层扫描 (SD-OCT) 确定体内角膜上皮的垂直和水平厚度分布。 SD-OCT 的组织轴向分辨率为 ∼3.3 µm，扫描深度更大。对 21 名受试者的 42 只眼睛进行了两次成像。评估整个水平和垂直角膜上皮厚度轮廓。分析了测试的重复性系数（CoR）和组内相关性（ICC）以及观察者间的变异性。检测到水平上皮的整个宽度，而对于覆盖的超眼睑则未显示部分上皮。第一次测量的平均中央上皮角膜厚度为 52.0±3.2 µm，第二次测量为 52.3±3.4 µm (P>.05)。在中心区（0-3.0毫米）、旁中心区（3.0-6.0毫米）和周围区（6.0-10.0毫米），平均上皮厚度范围分别为51至53微米、52至57微米和58至72微米。两次测试在双侧经络和左右眼上均无差异（P>.05）。两次测试的 ICC 范围为 0.70 至 0.97，CoR 从中心到外围的范围为 2.5 µm 至 7.8 µm，相当于 5.6% 至 10.6% (CoR%)。两名观察者的 ICC 从中心到外围的范围为 0.72 至 0.93，CoR 范围为 4.5 µm 至 10.4 µm，相当于 8.7% 至 15.2% (CoR%)。这项研究证明了超高分辨率和长扫描深度 SD-OCT 在评估角膜上皮的整个厚度轮廓方面具有良好的重复性。上皮厚度从中心向角膜缘增加。
To determine the vertical and horizontal thickness profiles of the corneal epithelium in vivo using ultra-long scan depth and ultra-high resolution spectral domain optical coherence tomography (SD-OCT). A SD-OCT was developed with an axial resolution of ∼3.3 µm in tissue and an extended scan depth. Forty-two eyes of 21 subjects were imaged twice. The entire horizontal and vertical corneal epithelial thickness profiles were evaluated. The coefficient of repeatability (CoR) and intraclass correlation (ICC) of the tests and interobserver variability were analyzed. The full width of the horizontal epithelium was detected, whereas part of the superior epithelium was not shown for the covered super eyelid. The mean central epithelial corneal thickness was 52.0±3.2 µm for the first measurement and 52.3±3.4 µm for the second measurement (P>.05). In the central zone (0–3.0 mm), the paracentral zones (3.0–6.0 mm) and the peripheral zones (6.0–10.0 mm), the mean epithelial thickness ranged from 51 to 53 µm, 52 to 57 µm, and 58 to 72 µm, respectively. There was no difference between the two tests at both meridians and in the right and left eyes (P>.05). The ICCs of the two tests ranged from 0.70 to 0.97 and the CoRs ranged from 2.5 µm to 7.8 µm from the center to the periphery, corresponding to 5.6% to 10.6% (CoR%). The ICCs of the two observers ranged from 0.72 to 0.93 and the CoRs ranged from 4.5 µm to 10.4 µm from the center to the periphery, corresponding to 8.7% to 15.2% (CoR%). This study demonstrated good repeatability of ultra-high resolution and long scan depth SD-OCT to evaluate the entire thickness profiles of the corneal epithelium. The epithelial thickness increases from the center toward the limbus.
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