Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels.
Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels.
复制标题
DOI：
10.1186/1479-5876-7-30
复制
发表时间：
2009-04-24
影响因子：
7.4
通讯作者：
Lurbe E
中科院分区：
文献类型：
--
作者：
Martín de Llano JJ;Fuertes G;Torró I;García Vicent C;Fayos JL;Lurbe E
低出生体重与成年后患高血压的风险增加有关。对脐带动脉和静脉的分子和细胞分析可以提供有关个体早期血管特征的信息。我们评估了不同出生体重的新生儿脐带来源的四种血管细胞类型的几种表型特征。进一步的后续研究可能会显示这些血管特性与婴儿期和成年期血压之间的关联。取两组出生体重小于2.8 kg和大于3.5 kg的新生儿脐带来源的血管内皮细胞和平滑肌细胞进行培养。对血管内皮细胞特异性标志物(von Willebrand因子，CD31，乙酰化低密度脂蛋白的结合和内化)和血管细胞特异性α-肌动蛋白的表达进行了评估。测定细胞培养活力、增殖动力学、生长分数(Ki67表达)和衰老细胞百分率(β-半乳糖苷酶在pH 6.0时的活性检测)。CD31免疫检测后，细胞培养形态计量学分析确定内皮细胞投射面积。脐动脉内皮细胞培养的细胞密度变异最大(66,789±5,093个/cm~2比45,630±11,927个/cm~2，p<；0.05)。形态计量学分析表明，动脉内皮细胞的投射面积(1,161±198和1,544±472μm~2，p<；0.05)小于出生体重>3.5 kg者的血管内皮细胞，而静脉内皮细胞的投射面积不低于出生体重>3.5 kg者。对脐带动脉内皮细胞的分析表明，细胞大小与出生体重有关，这可以为低出生体重与成人高血压风险增加之间的细胞和分子联系提供线索。
Low birth weight has been related to an increased risk for developing high blood pressure in adult life. The molecular and cellular analysis of umbilical cord artery and vein may provide information about the early vascular characteristics of an individual. We have assessed several phenotype characteristics of the four vascular cell types derived from human umbilical cords of newborns with different birth weight. Further follow-up studies could show the association of those vascular properties with infancy and adulthood blood pressure. Endothelial and smooth muscle cell cultures were obtained from umbilical cords from two groups of newborns of birth weight less than 2.8 kg or higher than 3.5 kg. The expression of specific endothelial cell markers (von Willebrand factor, CD31, and the binding and internalization of acetylated low-density lipoprotein) and the smooth muscle cell specific α-actin have been evaluated. Cell culture viability, proliferation kinetic, growth fraction (expression of Ki67) and percentage of senescent cells (detection of β-galactosidase activity at pH 6.0) have been determined. Endothelial cell projection area was determined by morphometric analysis of cell cultures after CD31 immunodetection. The highest variation was found in cell density at the confluence of endothelial cell cultures derived from umbilical cord arteries (66,789 ± 5,093 cells/cm2 vs. 45,630 ± 11,927 cells/cm2, p < 0.05). Morphometric analysis indicated that the projection area of the artery endothelial cells (1,161 ± 198 and 1,544 ± 472 μm2, p < 0.05), but not those derived from the vein from individuals with a birth weight lower than 2.8 kg was lower than that of cells from individuals with a birth weight higher than 3.5 kg. The analysis of umbilical cord artery endothelial cells, which demonstrated differences in cell size related to birth weight, can provide hints about the cellular and molecular links between lower birth weight and increased adult high blood pressure risk.
登录
查看更多内容
DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
影响因子：
--
作者：
OSMOND, C;BARKER, DJP;SIMMONDS, SJ
通讯作者：
SIMMONDS, SJ
影响因子：
15.9
作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
通讯作者：
MINICK, CR
影响因子：
105.7
作者：
BARKER, DJP;OSMOND, C
通讯作者：
OSMOND, C
影响因子：
4.9
作者：
Rummery, NM;McKenzie, KUS;Hill, CE
通讯作者：
Hill, CE