Coregulated human globin genes are frequently in spatial proximity when active.
Coregulated human globin genes are frequently in spatial proximity when active.
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DOI：
10.1083/jcb.200507073
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发表时间：
2006-01-16
期刊：
影响因子：
--
通讯作者：
Buckle VJ
中科院分区：
文献类型：
--
作者：
Brown JM;Leach J;Reittie JE;Atzberger A;Lee-Prudhoe J;Wood WG;Higgs DR;Iborra FJ;Buckle VJ
细胞核内基因的组织可能影响转录。我们已经分析了协调调节的α-和β-珠蛋白基因的核定位，并表明人类α-珠蛋白基因周围的基因密集染色质经常是解密集的，不依赖于转录。在此背景下，我们发现活跃的α-和β-珠蛋白基因和同源α-珠蛋白基因座的频繁并置发生在核斑点处，并与转录相关。然而，我们没有看到增加的信号共定位，这将是预期的直接物理相互作用。在人类β-珠蛋白基因之间或鼠珠蛋白基因之间不存在相同程度的接近性，这些基因更局限于它们的染色体区域。我们的研究结果表明，球蛋白基因在成红细胞核内的分布是自组织过程的结果，涉及转录状态、染色质的扩散能力以及与核蛋白的物理相互作用，而不是直接形式的高级控制。
The organization of genes within the nucleus may influence transcription. We have analyzed the nuclear positioning of the coordinately regulated α- and β-globin genes and show that the gene-dense chromatin surrounding the human α-globin genes is frequently decondensed, independent of transcription. Against this background, we show the frequent juxtaposition of active α- and β-globin genes and of homologous α-globin loci that occurs at nuclear speckles and correlates with transcription. However, we did not see increased colocalization of signals, which would be expected with direct physical interaction. The same degree of proximity does not occur between human β-globin genes or between murine globin genes, which are more constrained to their chromosome territories. Our findings suggest that the distribution of globin genes within erythroblast nuclei is the result of a self-organizing process, involving transcriptional status, diffusional ability of chromatin, and physical interactions with nuclear proteins, rather than a directed form of higher-order control.
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DOI：
10.1083/jcb.200207115
发表时间：
2002-12-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mahy NL;Perry PE;Bickmore WA
通讯作者：
Bickmore WA
影响因子：
2.3
作者：
Iborra, FJ
通讯作者：
Iborra, FJ
DOI：
10.1073/pnas.85.21.8081
发表时间：
1988-11-01
影响因子：
11.1
作者：
EPNER, E;FORRESTER, WC;GROUDINE, M
通讯作者：
GROUDINE, M
影响因子：
11.4
作者：
Gribnau, J;de Boer, E;Fraser, P
通讯作者：
Fraser, P
影响因子：
21.3
作者：
Brown, KE;Amoils, S;Fisher, AG
通讯作者：
Fisher, AG