Nanofibrous electrospun barrier membrane promotes osteogenic differentiation of human mesenchymal stem cells
Nanofibrous electrospun barrier membrane promotes osteogenic differentiation of human mesenchymal stem cells
复制标题
纳米纤维电纺屏障膜促进人间充质干细胞成骨分化
DOI：
10.1177/0883911511425297
复制
发表时间：
2011-11
影响因子：
1.7
通讯作者：
Man, Yi
中科院分区：
文献类型：
--
作者：
Wang, Ping;Gong, Ping;Lin, Yi;Qu, Yili;Li, Jidong;Kong, Xiangli;Chen, Zhiqing;Man, Yi
制备了一种电纺聚砜（PSU）作为引导性骨再生的屏障膜。该膜是纳米级的以防止纤维组织渗透，并且是高度多孔的以允许氧气和营养物渗透。观察人骨髓间充质干细胞（HBMSCs）的形态、贴壁、存活、增殖、分化及矿化情况。细胞在PSU膜上贴壁铺展良好，呈多角形、梭形和放射状延伸。活/死染色显示膜对细胞活力没有负面影响。与组织培养板相比，培养3 d后，PSU膜上的HBMSCs增殖率较低。然而，当细胞在PSU膜上培养时，分化活性特别以高水平表达。结果表明，PSU电纺膜能促进HBMSCs的成骨分化，具有良好的体外生物相容性，具有良好的屏障功能。
An electrospun polysulfone (PSU) was prepared as a barrier membrane for guided bone regeneration. The membrane was in nanoscale to prevent fibrous tissue infiltration and highly porous to allow permeation of oxygen and nutrients. The morphology and attachment, viability and proliferation, and differentiation and mineralization of human bone marrow mesenchymal stem cells (HBMSCs) were determined. Cells adhered and spread well on the PSU membrane with characteristic polygonal, fusiform shapes and radial extensions. The live/dead staining revealed that the membrane had no negative influence on cell viability. The proliferation rates of HBMSCs on PSU membranes were lower in comparison with tissue-culture polystyrene plate after 3 days of culture. However, differentiation activity was particularly expressed at high levels when cells were cultured on PSU membranes. The results based on the data suggest that the PSU electrospun membrane promoted the osteogenic differentiation of HBMSCs, displayed desirable in vitro biocompatibility, and has good potential as a barrier membrane.
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DOI：
10.1023/a:1008994601138
发表时间：
1998-12
期刊：
Journal of Materials Science: Materials in Medicine
影响因子：
--
作者：
F. Fey-Lamprecht;U. Gross;T. Groth;W. Albrecht;D. Paul;M. Fromm;A. Gitter
通讯作者：
F. Fey-Lamprecht;U. Gross;T. Groth;W. Albrecht;D. Paul;M. Fromm;A. Gitter
影响因子：
9.7
作者：
Vaquette, Cedryck;Cooper-White, Justin John
通讯作者：
Cooper-White, Justin John
影响因子：
14
作者：
Woo, Kyung Mi;Jun, Ji-Hae;Ma, Peter X.
通讯作者：
Ma, Peter X.
影响因子：
--
作者：
J. Attia;F. Legendre;Q. Nguyen;C. Baugé;K. Boumediene;J. Pujol
通讯作者：
J. Attia;F. Legendre;Q. Nguyen;C. Baugé;K. Boumediene;J. Pujol
影响因子：
14
作者：
Yoshimoto, H;Shin, YM;Vacanti, JP
通讯作者：
Vacanti, JP