Electrospun hydroxyapatite-containing chitosan nanofibers crosslinked with genipin for bone tissue engineering.
Electrospun hydroxyapatite-containing chitosan nanofibers crosslinked with genipin for bone tissue engineering.
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DOI：
10.1016/j.biomaterials.2012.09.009
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发表时间：
2012-12
期刊：
影响因子：
14
通讯作者：
Lelkes, Peter I.
中科院分区：
文献类型：
--
作者：
Frohbergh, Michael E.;Katsman, Anna;Botta, Gregory R.;Lazarovici, Phillip;Schauer, Caroline L.;Wegst, Ulrike G. K.;Lelkes, Peter I.
大骨缺损的重建在骨科和颅面临床实践中仍然存在问题。自体移植物的供应有限，并且与供体部位的发病率有关，而其他材料与宿主自身骨骼的整合性较差。这种缺乏整合通常是由于缺乏骨膜，骨膜是骨骼的外层，含有骨祖细胞，对骨组织的生长和重塑至关重要。在这项研究中，我们开发了一种一步式平台，可以用壳聚糖静电纺丝纳米纤维支架，该支架还含有羟基磷灰石纳米颗粒，并与京尼平交联。我们假设所得的复合支架代表了一种微环境，该微环境模拟非承重骨细胞外基质的物理、矿化结构和机械特性，同时促进类似于骨膜的成骨细胞分化和成熟。使用扫描电子显微镜和光谱技术研究了支架的超微结构和物理化学性质。电纺支架的平均纤维直径在纺丝时为227±154 nm，与京尼平交联后增加至335±119 nm。 X射线衍射、傅里叶变换红外光谱和能量色散光谱分析证实复合壳聚糖纤维中存在羟基磷灰石特征。复合纤维支架的杨氏模量为142±13 MPa，与天然骨膜的杨氏模量相似。纯壳聚糖支架和含羟基磷灰石复合壳聚糖支架均支持小鼠7F2成骨细胞样细胞的粘附、增殖和成骨分化。与纯壳聚糖支架相比，在复合支架上培养的细胞中碱性磷酸酶（一种早期成骨标志物）的表达和酶活性更高，到第 14 天时达到显着的 2.4 倍差异（p<0.05）。同样，在含有羟基磷灰石的支架上培养的细胞在两周内具有最高的骨连接蛋白 mRNA 表达率，表明复合支架的骨诱导性增强。我们的结果表明，将含羟基磷灰石的静电纺丝壳聚糖与京尼平交联可产生生物复合支架，它将非承重骨机械性能与骨膜样环境相结合，并促进成骨细胞样细胞的增殖、分化和成熟。我们认为这些支架可能有助于颌面部缺陷和损伤的修复和再生。
Reconstruction of large bone defects remains problematic in orthopedic and craniofacial clinical practice. Autografts are limited in supply and are associated with donor site morbidity while other materials show poor integration with the host’s own bone. This lack of integration is often due to the absence of periosteum, the outer layer of bone that contains osteoprogenitor cells and is critical for the growth and remodeling of bone tissue. In this study we developed a one-step platform to electrospin nanofibrous scaffolds from chitosan, which also contain hydroxyapatite nanoparticles and are crosslinked with genipin. We hypothesized that the resulting composite scaffolds represent a microenvironment that emulates the physical, mineralized structure and mechanical properties of non-weight bearing bone extracellular matrix while promoting osteoblast differentiation and maturation similar to the periosteum. The ultrastructure and physicochemical properties of the scaffolds were studied using scanning electron microscopy and spectroscopic techniques. The average fiber diameters of the electrospun scaffolds were 227±154 nm as spun, and increased to 335±119 nm after crosslinking with genipin. Analysis by X-ray diffraction, Fourier transformed infrared spectroscopy and energy dispersive spectroscopy confirmed the presence of characteristic features of hydroxyapatite in the composite chitosan fibers. The Young’s modulus of the composite fibrous scaffolds was 142±13 MPa, which is similar to that of the natural periosteum. Both pure chitosan scaffolds and composite hydroxyapatite-containing chitosan scaffolds supported adhesion, proliferation and osteogenic differentiation of mouse 7F2 osteoblast-like cells. Expression and enzymatic activity of alkaline phosphatase, an early osteogenic marker, were higher in cells cultured on the composite scaffolds as compared to pure chitosan scaffolds, reaching a significant, 2.4 fold, difference by day 14 (p<0.05). Similarly, cells cultured on hydroxyapatite-containing scaffolds had the highest rate of osteonectin mRNA expression over 2 weeks, indicating enhanced osteoinductivity of the composite scaffolds. Our results suggest that crosslinking electrospun hydroxyapatite-containing chitosan with genipin yields bio-composite scaffolds, which combine non-weight-bearing bone mechanical properties with a periosteum-like environment and facilitate the proliferation, differentiation and maturation of osteoblast-like cells. We propose that these scaffolds might be useful for the repair and regeneration of maxillofacial defects and injuries.
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影响因子：
14
作者：
Geng, XY;Kwon, OH;Jang, JH
通讯作者：
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DOI：
10.1016/j.ijbiomac.2010.09.017
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作者：
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