Virological and molecular characterization of a mammalian orthoreovirus type 3 strain isolated from a dog in Italy.
Virological and molecular characterization of a mammalian orthoreovirus type 3 strain isolated from a dog in Italy.
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DOI：
10.1016/j.vetmic.2005.05.014
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发表时间：
2005-08-10
影响因子：
3.3
通讯作者：
Buonavoglia C
中科院分区：
文献类型：
--
作者：
Decaro N;Campolo M;Desario C;Ricci D;Camero M;Lorusso E;Elia G;Lavazza A;Martella V;Buonavoglia C
从一只同时感染犬细小病毒2型的致死性腹泻仔犬中分离到一株哺乳动物正病毒(MRV)。该病毒分离株具有非典型的血凝模式和S1片段的电泳率降低，这是MRV 3型的特征。通过针对S1基因的类型特异性RT-PCR检测和随后的序列分析，确认分离出的病毒属于MRV-3。根据几个MRV的S1基因的系统发育分析，该分离株与小鼠株T3C9/61、牛株T3C18/61和T3C31/59一起属于E系。相反，发现无论病毒类型如何，L1序列都是分离的。对110份腹泻或鼻/眼分泌物犬的粪便样本、56份鼻拭子和31份眼拭子进行了针对呼肠孤病毒的套式聚合酶链式反应检测，未发现含有MRV RNA的样本，这一发现与犬的血清阳性率(25.77%)明显相反。
A mammalian orthoreovirus (MRV) strain was isolated from a pup with fatal diarrhea, which had a concurrent infection by canine parvovirus type 2. The reovirus isolate showed an atypical hemagglutination pattern and a retarded electrophoretic mobility of the S1 segment, which is characteristic of MRV type 3 (MRV-3). Assignment of the isolated virus to MRV-3 was confirmed by type-specific RT-PCR assays, targeting the S1 gene, and by subsequent sequence analysis of the PCR product. By phylogeny based on the S1 gene of several MRVs, the isolate fell into lineage E, along with the murine strain T3C9/61 and the bovine strains T3C18/61 and T3C31/59. Conversely, L1 sequences were found to segregate regardless of the viral type. A total of 110 fecal samples, 56 nasal and 31 ocular swabs from dogs with diarrhea or nasal/ocular discharge were tested by a nested-PCR assay specific for reoviruses, and no sample was found to contain MRV RNA, a finding that is apparently in contrast with the seroprevalence (25.77%) observed in dogs.
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影响因子：
5.4
作者：
FRASER, RDB;FURLONG, DB;STEVEN, AC
通讯作者：
STEVEN, AC
影响因子：
5.4
作者：
DERMODY, TS;NIBERT, ML;FIELDS, BN
通讯作者：
FIELDS, BN
DOI：
10.1073/pnas.82.1.24
发表时间：
1985-01-01
影响因子：
11.1
作者：
CASHDOLLAR, LW;CHMELO, RA;JOKLIK, WK
通讯作者：
JOKLIK, WK
影响因子：
5.4
作者：
BASSELDUBY, R;SPRIGGS, DR;FIELDS, BN
通讯作者：
FIELDS, BN
影响因子：
5
作者：
HOLZINGER, EA;GRIESEMER, RA
通讯作者：
GRIESEMER, RA