Pinealectomy affects bone mineral density and structure--an experimental study in sheep.
Pinealectomy affects bone mineral density and structure--an experimental study in sheep.
复制标题
DOI：
10.1186/1471-2474-12-271
复制
发表时间：
2011-11-24
影响因子：
2.3
通讯作者：
Alini M
中科院分区：
文献类型：
--
作者：
Egermann M;Gerhardt C;Barth A;Maestroni GJ;Schneider E;Alini M
骨质疏松症和相关的骨折是一个主要的公共卫生负担，非常需要一个大型动物模型。褪黑激素，松果体的激素，已被证明影响骨代谢。本研究的目的是评估是否缺乏褪黑激素由于松果体切除影响骨量，结构和改建的绵羊动物模型。将雌性绵羊分为对照组、手术切除卵巢组（Ovx）、手术切除松果体组（Px）和Ovx+Px组。术前和术后6个月，采集髂嵴活检组织，并使用μCT测量结构参数。测定骨形成和骨吸收的标志物。为了评估松果体切除术后的长期变化，在0、3、9、18和30个月时分析桡骨远端的骨矿物质密度（BMD）。6个月后松质骨体积（BV/TV）下降了-13.3%Px和21.5%OvxPx。骨丢失是由于骨小梁分离增加以及厚度减少。组织形态定量和胶原降解产物的测定显示松果体切除术后骨吸收增加。单独卵巢切除术导致桡骨远端一过性骨丢失，随后持续增加至基线水平。松果体切除术后的骨吸收活动导致骨丢失，这不是短暂的，因为观察到BMD持续下降，直到30个月。羊松果体切除术后的变化是骨质流失的指示。总的来说，这些研究结果表明，松果体可能会影响骨代谢，松果体切除术可用于诱导羊骨丢失。
Osteoporosis and associated fractures are a major public health burden and there is great need for a large animal model. Melatonin, the hormone of the pineal gland, has been shown to influence bone metabolism. This study aims to evaluate whether absence of melatonin due to pinealectomy affects the bone mass, structure and remodeling in an ovine animal model. Female sheep were arranged into four groups: Control, surgically ovariectomized (Ovx), surgically pinealectomized (Px) and Ovx+Px. Before and 6 months after surgery, iliac crest biopsies were harvested and structural parameters were measured using μCT. Markers of bone formation and resorption were determined. To evaluate long term changes after pinealectomy, bone mineral density (BMD) was analyzed at the distal radius at 0, 3, 9, 18 and 30 months. Cancellous bone volume (BV/TV) declined after 6 months by -13.3% Px and -21.5% OvxPx. The bone loss was due to increased trabecular separation as well as decreased thickness. The histomorphometric quantification and determination of collagen degradation products showed increased bone resorption following pinealectomy. Ovariectomy alone results in a transient bone loss at the distal radius followed by continuous increase to baseline levels. The bone resorption activity after pinealectomy causes a bone loss which was not transient, since a continuous decrease in BMD was observed until 30 months. The changes after pinealectomy in sheep are indicative of bone loss. Overall, these findings suggest that the pineal gland may influence bone metabolism and that pinealectomy can be used to induce bone loss in sheep.
登录
查看更多内容
影响因子：
2.3
作者：
BARRIOS, C;BROSTROM, LA;WALHEIM, G
通讯作者：
WALHEIM, G
影响因子：
3.3
作者：
Sigrist, Ilonka M.;Gerhardt, Christian;Egermann, Marcus
通讯作者：
Egermann, Marcus
影响因子：
6.1
作者：
Ladizesky, MG;Cutrera, RA;Cardinali, DP
通讯作者：
Cardinali, DP
影响因子：
10.3
作者：
Conti, A;Conconi, S;Maestroni, GJM
通讯作者：
Maestroni, GJM
影响因子：
--
作者：
DEMPSEY, RJ;HOPKINS, J;KINDT, GW
通讯作者：
KINDT, GW