Comparison of natural crosslinking agents for the stabilization of xenogenic articular cartilage.
Comparison of natural crosslinking agents for the stabilization of xenogenic articular cartilage.
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DOI：
10.1002/jor.23121
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Elder S
中科院分区：
文献类型：
--
作者：
Pinheiro A;Cooley A;Liao J;Prabhu R;Elder S
骨软骨异种移植物是潜在的廉价，广泛可用的替代新鲜同种异体移植物。然而，从异种软骨中去除抗原可能会破坏细胞外基质并降低压缩刚度。非交联异种软骨也可能在体内经历快速酶降解。我们假设天然交联剂可以代替戊二醛来改善脱细胞软骨的机械性能和酶抗性。本研究比较了京尼平（GNP）、原花青素（PA）和表没食子儿茶素没食子酸酯（EGCG）对脱细胞猪软骨物理和机械性能的影响。戊二醛（GA）作为阳性对照。将猪关节软骨盘在2%十二烷基硫酸钠和DNA酶I中脱细胞，然后在0.25%GNP、0.25%PA、0.25%EGCG或2.5%GA中固定。去细胞化使DNA减少15%，GAG减少35%。对于天然交联剂，平均交联度范围为约50%（EGCG）至78%（GNP），而GA对照为83%。在天然交联剂中，只有GNP显著影响盘直径，并且收缩率低于2%。GA固定对椎间盘直径无显著影响。去细胞化降低了聚集体模量; GA和GNP，但不是EGCG和PA，能够将其恢复到其原始水平。GNP，PA和GA赋予了类似的，几乎完全抵抗胶原酶降解。EGCG也赋予实质性的抵抗力，但程度较低。总的来说，数据支持我们的假设，并表明天然交联剂可能是戊二醛的合适替代品，用于稳定脱细胞软骨。
Osteochondral xenografts are potentially inexpensive, widely available alternatives to fresh allografts. However, antigen removal from xenogenic cartilage may damage the extracellular matrix and reduce compressive stiffness. Non-crosslinked xenogenic cartilage may also undergo rapid enzymatic degradation in vivo. We hypothesized that natural crosslinking agents could be used in place of glutaraldehyde to improve the mechanical properties and enzymatic resistance of decellularized cartilage. This study compared the effects of genipin (GNP), proanthocyanidin (PA), and epigallocatechin gallate (EGCG), on the physical and mechanical properties of decellularized porcine cartilage. Glutaraldehyde (GA) served as a positive control. Porcine articular cartilage discs were decellularized in 2% sodium dodecyl sulfate and DNase I followed by fixation in 0.25% GNP, 0.25% PA, 0.25% EGCG, or 2.5% GA. Decellularization decreased DNA by 15% and GAG by 35%. For natural crosslinkers, the average degree of crosslinking ranged from approximately 50% (EGCG) to 78% (GNP), as compared to 83% for the GA control. Among the natural crosslinkers, only GNP significantly affected the disc diameter, and shrinkage was under 2%. GA fixation had no significant effect on disc diameter. Decellularization decreased aggregate modulus; GA and GNP, but not EGCG and PA, were able to restore it to its original level. GNP, PA and GA conferred a similar, almost complete resistance to collagenase degradation. EGCG also conferred substantial resistance but to a lesser degree. Overall, the data support our hypothesis and suggest that natural crosslinkers may be suitable alternatives to glutaraldehyde for stabilization of decellularized cartilage.
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DOI：
10.1097/01.blo.0000164298.63534.64
发表时间：
2005-07-01
影响因子：
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作者：
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