Stomach antral endocrine cells in patients with irritable bowel syndrome.
Stomach antral endocrine cells in patients with irritable bowel syndrome.
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DOI：
10.3892/ijmm.2014.1887
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发表时间：
2014-10
影响因子：
5.4
通讯作者：
Hausken T
中科院分区：
文献类型：
--
作者：
El-Salhy M;Gilja OH;Hatlebakk JG;Hausken T
据我们所知，胃窦内分泌细胞以前没有研究过肠易激综合征（IBS）患者。因此，在本研究中，检查了76名IBS患者（指定为IBS-总）。其中26例患者（IBS-D）的主要症状是腹泻，而21例患者的主要症状是腹泻和便秘（IBS-M），29例患者的主要症状是便秘（IBS-C）。43例健康受试者作为对照。从所有受试者获得的胃窦活检样本进行免疫染色，使用抗生物素蛋白-生物素复合物法的血清素，胃泌素，生长抑素和血清素转运蛋白（SERT）。免疫阳性细胞密度和免疫反应强度通过计算机辅助图像分析确定。与对照组相比，IBS-M患者的降钙素免疫反应阳性细胞密度显著降低，IBS-C患者的降钙素免疫反应阳性细胞密度显著升高。免疫反应强度在对照组和IBS-总组之间没有显著差异。IBS-D、IBS-M和IBS-C患者的胃泌素免疫反应细胞密度显著高于对照组。胃泌素的免疫反应强度在IBS-D患者中显著高于对照组。总IBS、IBS-D、IBS-M和IBS-C患者的生长激素抑制素免疫反应细胞密度明显低于对照组。生长抑素和SERT的免疫反应强度在对照组和IBS组之间没有显着差异。所有IBS亚型中胃泌素细胞密度的增加和生长抑素细胞密度的降低可能导致高水平的胃分泌，这可能反过来导致在IBS患者中观察到的消化不良和胃食管反流的高发生率。
To the best of our knowledge, stomach antral endocrine cells have not previously been investigated in patients with irritable bowel syndrome (IBS). Thus, in the present study, 76 patients with IBS were examined (designated as IBS-total). Diarrhoea was the predominant symptom in 26 of these patients (IBS-D), while in 21 patients, the predominant symptoms were both diarrhoea and constipation (IBS-M) and in 29 patients the predominant symptom was constipation (IBS-C). Forty-three healthy subjects were enrolled as the controls. Stomach antral biopsy samples obtained from all of the subjects were immunostained using the avidin-biotin-complex method for serotonin, gastrin, somatostatin and serotonin transporter (SERT). The immunopositive cell densities and immunoreactivity intensities were determined by computer-aided image analysis. The density of the serotonin-immunoreactive cells was significantly decreased in the IBS-M patients and increased in the IBS-C patients relative to the controls. The immunoreactivity intensity did not differ significantly between the controls and IBS-total. The density of the gastrin-immunoreactive cells was significantly greater in the IBS-D, IBS-M and IBS-C patients than in the controls. The immunoreactivity intensity of gastrin was significantly greater in the IBS-D patients than in the controls. The density of the somatostatin-immunoreactive cells cells was significantly lower in the IBS-total, IBS-D, IBS-M and IBS-C patients than in the controls. The immunoreactivity intensities of both somatostatin and SERT did not differ significantly between the controls and IBS-total. The increase in gastrin cell density and the decrease in somatostatin cell density in all IBS subtypes may cause high levels of gastric secretion, which may in turn contribute to the high incidence of dyspepsia and gastro-oesophageal reflux observed in patients with IBS.
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影响因子：
4.3
作者：
El-Salhy, Magdy;Gundersen, Doris;Hausken, Trygve
通讯作者：
Hausken, Trygve
影响因子：
3.1
作者：
El-Salhy, Magdy;Vaali, K.;Hausken, T.
通讯作者：
Hausken, T.
影响因子：
5.4
作者：
El-Salhy, M.;Ostgaard, H.;Hausken, T.
通讯作者：
Hausken, T.
DOI：
10.1097/med.0b013e32835bc703
发表时间：
2013-02
期刊：
Current opinion in endocrinology, diabetes, and obesity
影响因子：
--
作者：
Gershon MD
通讯作者：
Gershon MD
影响因子：
3.4
作者：
El-Salhy, Magdy;Wendelbo, Ingvild;Gundersen, Doris
通讯作者：
Gundersen, Doris