Postembryonic neuronal addition in zebrafish dorsal root ganglia is regulated by Notch signaling.
Postembryonic neuronal addition in zebrafish dorsal root ganglia is regulated by Notch signaling.
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DOI：
10.1186/1749-8104-7-23
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发表时间：
2012-06-27
影响因子：
3.6
通讯作者：
Raible DW
中科院分区：
文献类型：
--
作者：
McGraw HF;Snelson CD;Prendergast A;Suli A;Raible DW
背根神经节（DRG）的感觉神经元和神经胶质是由发育中的脊椎动物胚胎中的神经嵴细胞产生的。在小鼠和鸡中，DRG的形成在胚胎发生时完成。相比之下，斑马鱼在神经嵴迁移完成后很长一段时间内仍继续向DRG添加神经元和胶质细胞。斑马鱼DRG晚期生长的分子和细胞调控仍有待研究。在本研究中，我们使用转基因斑马鱼系来检测胚胎后DRG生长过程中的神经元添加。在幼虫发育期间，神经元的增加是连续的。命运映射实验支持这样一种假设，即新的神经元是从一群常驻的、神经嵴衍生的祖细胞中添加的。我们使用Notch信号的条件抑制来评估该信号通路在神经元添加中的作用。当Notch信号在幼虫发育的早期和晚期被抑制时，DRG神经元的数量会增加。斑马鱼DRG的胚胎后生长在一定程度上是由于来自原有祖细胞群的新神经元的增加，这一过程受到Notch信号的调节。
The sensory neurons and glia of the dorsal root ganglia (DRG) arise from neural crest cells in the developing vertebrate embryo. In mouse and chick, DRG formation is completed during embryogenesis. In contrast, zebrafish continue to add neurons and glia to the DRG into adulthood, long after neural crest migration is complete. The molecular and cellular regulation of late DRG growth in the zebrafish remains to be characterized. In the present study, we use transgenic zebrafish lines to examine neuronal addition during postembryonic DRG growth. Neuronal addition is continuous over the period of larval development. Fate-mapping experiments support the hypothesis that new neurons are added from a population of resident, neural crest-derived progenitor cells. Conditional inhibition of Notch signaling was used to assess the role of this signaling pathway in neuronal addition. An increase in the number of DRG neurons is seen when Notch signaling is inhibited during both early and late larval development. Postembryonic growth of the zebrafish DRG comes about, in part, by addition of new neurons from a resident progenitor population, a process regulated by Notch signaling.
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DOI：
10.1007/s10162-002-3022-x
发表时间：
2003-06-01
影响因子：
2.4
作者：
Harris, JA;Cheng, AG;Rubel, EW
通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
Ernfors, Patrik
影响因子：
2.7
作者：
Gray, M;Moens, CB;Beattie, CE
通讯作者：
Beattie, CE