Individual signatures and environmental factors shape skin microbiota in healthy dogs.
Individual signatures and environmental factors shape skin microbiota in healthy dogs.
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DOI：
10.1186/s40168-017-0355-6
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发表时间：
2017-10-13
期刊：
影响因子：
15.5
通讯作者：
Francino O
中科院分区：
文献类型：
--
作者：
Cuscó A;Belanger JM;Gershony L;Islas-Trejo A;Levy K;Medrano JF;Sánchez A;Oberbauer AM;Francino O
据报道，个人及其环境是驱动健康狗皮肤微生物群组成和结构的主要力量。因此，分析犬皮肤微生物群时的主要问题之一是环境的可能影响。尽管覆盖着浓密的皮毛，但某些皮肤病在皮肤部位、犬种和个体之间表现出不同的患病率。我们已经在一个控制良好的队列中描述了狗皮肤微生物群的正常变异性，该队列包括大量具有相似年龄、相关遗传背景和共享环境的金拉布拉多猎犬杂交犬（N = 35）。我们发现，个人驱动皮肤微生物群的组成和结构，然后是皮肤部位。在该队列中，居住在狗皮肤上的主要细菌类别是γ-变形菌门和杆菌。我们还在不同的狗皮肤部位检测到与环境相关的细菌，这可能反映了每个皮肤部位和每只狗的不同暴露程度。网络分析阐明了皮肤部位内和皮肤部位之间的细菌相互作用，特别是在下巴、腹部、腋窝和肛周区域，高度共享的相互作用可能代表解剖学、行为或环境成分。当独立分析每个皮肤部位以评估宿主特异性因素时，我们发现时间性（出生季节和在犬舍中度过的时间）影响所有皮肤部位，特别是内耳廓。造成这种差异的最丰富的分类群是鞘氨醇单胞菌属。我们还发现了雄性和雌性狗之间的腹部，腋窝和背部的分类差异。我们观察到皮肤部位之间存在较大的个体间变异性和差异。特定于宿主的变量，如时间性或性别，也会影响健康狗的皮肤微生物群，即使在环境同质的队列中也是如此。本文的在线版本（10.1186/s40168-017-0355-6）包含补充材料，可供授权用户使用。
The individual, together with its environment, has been reported as the main force driving composition and structure of skin microbiota in healthy dogs. Therefore, one of the major concerns when analyzing canine skin microbiota is the likely influence of the environment. Despite the dense fur covering, certain skin diseases exhibit differential prevalence among skin sites, dog breeds, and individuals. We have characterized the normal variability of dog skin microbiota in a well-controlled cohort of a large number of Golden-Labrador Retriever crossed dogs (N = 35) with similar ages, related genetic background, and a shared environment. We found that the individual drives the skin microbiota composition and structure followed by the skin site. The main bacterial classes inhabiting dog skin in this cohort are Gammaproteobacteria and Bacilli. We also detected bacteria associated to the environment on different dog skin sites that could be reflecting the different degrees of exposure of each skin site and each dog. Network analyses elucidated bacterial interactions within and between skin sites, especially in the chin, abdomen, axilla, and perianal region, with the highly shared interactions probably representing an anatomical, behavioral, or environmental component. When analyzing each skin site independently to assess host-specific factors, we found that temporality (season of birth and time spent in the kennel) affected all the skin sites and specially the inner pinna. The most abundant taxon driving this difference was Sphingomonas. We also found taxonomic differences among male and female dogs on the abdomen, axilla, and back. We observed a large inter-individual variability and differences among skin sites. Host-specific variables, such as temporality or sex, were also shaping skin microbiota of healthy dogs, even in an environmental homogenous cohort. The online version of this article (10.1186/s40168-017-0355-6) contains supplementary material, which is available to authorized users.
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DOI：
10.1126/science.1224203
发表时间：
2012-06-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Costello EK;Stagaman K;Dethlefsen L;Bohannan BJ;Relman DA
通讯作者：
Relman DA
影响因子：
6.5
作者：
Capone, Kimberly A.;Dowd, Scot E.;Stamatas, Georgios N.;Nikolovski, Janeta
通讯作者：
Nikolovski, Janeta
影响因子：
4.3
作者：
Faust K;Sathirapongsasuti JF;Izard J;Segata N;Gevers D;Raes J;Huttenhower C
通讯作者：
Huttenhower C
DOI：
10.1073/pnas.1205624109
发表时间：
2012-05-22
影响因子：
11.1
作者：
Hanski, Ilkka;von Hertzen, Leena;Haahtela, Tari
通讯作者：
Haahtela, Tari
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob