Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells.
Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells.
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DOI：
10.1038/s41598-021-94384-1
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发表时间：
2021-07-22
影响因子：
4.6
通讯作者：
Kudo Y
中科院分区：
文献类型：
--
作者：
Shao W;Fujiwara N;Mouri Y;Kisoda S;Yoshida K;Yoshida K;Yumoto H;Ozaki K;Ishimaru N;Kudo Y
癌细胞经历部分上皮间质转化（p-EMT）而不是完全EMT的能力会带来更高的转移风险。尽管具核梭杆菌主要栖息在口腔中，但人们的注意力集中在具核梭杆菌与结直肠癌发展的关系上。在这里，我们研究了具核梭菌对口腔鳞状细胞癌 (OSCC) 细胞的 p-EMT 调节。我们用活的或热灭活的具核梭杆菌培养具有上皮细胞、p-EMT 或 EMT 表型的 OSCC 细胞。在与具核梭菌共培养后，通过 qPCR 检查 OSCC 细胞中参与上皮分化、p-EMT 和 EMT 的基因的表达。还检查了 OSCC 细胞的细胞生长和侵袭。活的和热灭活的具核梭杆菌均上调具有上皮表型的 OSCC 细胞中 p-EMT 相关基因的表达，但不上调 p-EMT 或 EMT 表型的 OSCC 细胞。此外，具核梭菌促进具有上皮表型的 OSCC 细胞的侵袭。与牙龈卟啉单胞菌以外的其他细菌菌株共培养不会改变具有上皮表型的 OSCC 细胞中的 p-EMT 相关基因。具核梭菌感染可能通过改变 OSCC 中的基因表达将上皮细胞表型转化为 p-EMT 表型。针对具核梭菌感染的口腔卫生管理可能有助于降低 OSCC 转移能力增加的风险。
The ability of cancer cells to undergo partial-epithelial mesenchymal transition (p-EMT), rather than complete EMT, poses a higher metastatic risk. Although Fusobacterium nucleatum mainly inhabits in oral cavity, attention has been focused on the F. nucleatum involvement in colorectal cancer development. Here we examined the p-EMT regulation by F. nucleatum in oral squamous cell carcinoma (OSCC) cells. We cultured OSCC cells with epithelial, p-EMT or EMT phenotype with live or heat-inactivated F. nucleatum. Expression of the genes involved in epithelial differentiation, p-EMT and EMT were examined in OSCC cells after co-culture with F. nucleatum by qPCR. Cell growth and invasion of OSCC cells were also examined. Both live and heat-inactivated F. nucleatum upregulated the expression of p-EMT-related genes in OSCC cells with epithelial phenotype, but not with p-EMT or EMT phenotype. Moreover, F. nucleatum promoted invasion of OSCC cells with epithelial phenotype. Co-culture with other strains of bacteria other than Porphyromonas gingivalis did not alter p-EMT-related genes in OSCC cells with epithelial phenotype. F. nucleatum infection may convert epithelial to p-EMT phenotype via altering gene expression in OSCC. Oral hygiene managements against F. nucleatum infection may contribute to reduce the risk for an increase in metastatic ability of OSCC.
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影响因子：
4.5
作者：
Harrandah AM;Chukkapalli SS;Bhattacharyya I;Progulske-Fox A;Chan EKL
通讯作者：
Chan EKL
影响因子：
3.1
作者：
Han, YW;Shi, WY;Genco, RJ
通讯作者：
Genco, RJ
影响因子：
7
作者：
Castellarin, Mauro;Warren, Rene L.;Holt, Robert A.
通讯作者：
Holt, Robert A.
影响因子：
3.7
作者：
Anand, Preetha;Kunnumakara, Ajaikumar B.;Sundaram, Chitra;Harikumar, Kuzhuvelil B.;Tharakan, Sheeja T.;Lai, Oiki S.;Sung, Bokyung;Aggarwal, Bharat B.
通讯作者：
Aggarwal, Bharat B.
影响因子：
32.4
作者：
Gur, Chamutal;Ibrahim, Yara;Isaacson, Batya;Yamin, Rachel;Abed, Jawad;Gamliel, Moriya;Enk, Jonatan;Bar-On, Yotam;Stanietsky-Kaynan, Noah;Coppenhagen-Glazer, Shunit;Shussman, Noam;Almogy, Gideon;Cuapio, Angelica;Hofer, Erhard;Mevorach, Dror;Tabib, Adi;Ortenberg, Rona;Markel, Gal;Miklic, Karmela;Jonjic, Stipan;Brennan, Caitlin A.;Garrett, Wendy S.;Bachrach, Gilad;Mandelboim, Ofer
通讯作者：
Mandelboim, Ofer