Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein.
Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein.
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DOI：
10.1083/jcb.99.6.2034
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发表时间：
1984-12
期刊：
影响因子：
--
通讯作者：
Zetter BR
中科院分区：
文献类型：
--
作者：
Voyta JC;Via DP;Butterfield CE;Zetter BR
乙酰化低密度脂蛋白（Ac-LDL）通过LDL代谢的“清道夫细胞途径”被巨噬细胞和内皮细胞摄取。在这份报告中，主动脉和微血管内皮细胞内化和降解的[125 I]-Ac-LDL是平滑肌细胞或周细胞的7-15倍。结合的[125 I]-Ac-LDL被未标记的Ac-LDL取代，而不是未修饰的LDL。用荧光探针1，1 ′-双十八烷基-3，3，3 ′，3 ′-四甲基吲哚羰花青高氯酸盐（Dil-Ac- LDL）标记的Ac-LDL检测了基于Ac-LDL代谢增加的内皮细胞的鉴定能力。当细胞与10微克/毫升Dil-Ac-LDL在37 ℃下孵育4小时，随后通过荧光显微镜检查时，毛细血管和主动脉内皮细胞发出明亮的荧光，而视网膜周细胞和平滑肌细胞的荧光强度仅略高于背景水平。用于标记细胞的浓度的Dil-Ac-LDL对内皮细胞生长速率没有影响。当牛肾上腺毛细血管细胞的原代培养物用10微克/毫升的Dil-Ac-LDL在37 ℃标记4小时，然后胰蛋白酶消化并进行荧光激活细胞分选时，可以获得毛细血管内皮细胞的纯培养物。利用这种方法，大量的早期传代微血管内皮细胞，可以在显着更少的时间比传统的方法。
Acetylated-low density lipoprotein (Ac-LDL) is taken up by macrophages and endothelial cells via the "scavenger cell pathway" of LDL metabolism. In this report, aortic and microvascular endothelial cells internalized and degraded 7-15 times more [125I]-Ac-LDL than did smooth muscle cells or pericytes. Bound [125I]-Ac-LDL was displaced by unlabeled Ac-LDL, but not unmodified LDL. The ability to identify endothelial cells based on their increased metabolism of Ac-LDL was examined using Ac-LDL labeled with the fluorescent probe 1,1'- dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (Dil-Ac- LDL). When cells were incubated with 10 micrograms/ml Dil-Ac-LDL for 4 h at 37 degrees C and subsequently examined by fluorescence microscopy, capillary and aortic endothelial cells were brilliantly fluorescent whereas the fluorescent intensity of retinal pericytes and smooth muscle cells was only slightly above background levels. Dil-Ac-LDL at the concentration used for labeling cells had no effect on endothelial cell growth rate. When primary cultures of bovine adrenal capillary cells were labeled with 10 micrograms/ml of Dil-Ac-LDL for 4 h at 37 degrees C, then trypsinized and subjected to fluorescence-activated cell sorting, pure cultures of capillary endothelial cells could be obtained. Utilizing this method, large numbers of early passage microvascular endothelial cells can be obtained in significantly less time than with conventional methods.
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影响因子：
5.6
作者：
DELVECCHIO, PJ;SMITH, JR
通讯作者：
SMITH, JR
影响因子：
9
作者：
HERRING, MB;DILLEY, R;GLOVER, J
通讯作者：
GLOVER, J
DOI：
10.1016/0005-2760(80)90155-1
发表时间：
1980-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
STEIN, O;STEIN, Y
通讯作者：
STEIN, Y
DOI：
10.1073/pnas.76.10.5217
发表时间：
1979-01-01
影响因子：
11.1
作者：
FOLKMAN, J;HAUDENSCHILD, CC;ZETTER, BR
通讯作者：
ZETTER, BR
影响因子：
15.9
作者：
HOYER, LW;SANTOS, RPD;HOYER, JR
通讯作者：
HOYER, JR