Screening, isolation and molecular identification of biodegrading mycobacteria from Iranian ecosystems and analysis of their biodegradation activity.
Screening, isolation and molecular identification of biodegrading mycobacteria from Iranian ecosystems and analysis of their biodegradation activity.
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DOI：
10.1186/s13568-017-0472-4
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发表时间：
2017-09-20
期刊：
影响因子：
3.7
通讯作者：
Naser AD
中科院分区：
文献类型：
--
作者：
Azadi D;Shojaei H;Mobasherizadeh S;Naser AD
人为来源的污染物，包括农药，重金属，药物和工业化学品对人类健康和环境造成许多风险，生物修复被认为是减少危险化学品风险的首选策略。在目前的研究中，我们的目的是筛选和表征分枝杆菌从不同范围的伊朗水生和陆地生态系统的恶劣和不利的环境条件，可用于目标污染物的生物降解。从90个环境样品中分离出分枝杆菌，并使用常规的微生物和分子方法，包括PCR扩增hsp 65和序列分析，16 S rRNA遗传标记鉴定到种水平。采用生长速率法、色谱法、吉布斯法和浊度法对菌株的生物降解活性进行了评价。从90份标本中分离出39株分枝杆菌，占43.3%，分属于21个种，其中M. 6个分离物，M. flavescens和M. paragordonae，M. monacense，M. fredriksbergense和M. aurum;各2个分离物; conceptionense，M. porcinum、M. simiae、M. celeriflavum，M. novocastrense，M. neoaurum，M. obuense和12个分离株，属于8个未知的潜在新分枝杆菌种。根据其生物修复活性，将分离物分为三组，即，5株（12.8%）无生物降解活性，20株（51.2%）有生物降解活性，14株（35.9%）有生物降解活性但未报道。我们的研究结果表明，伊朗的生态系统拥有一个良好的水库不同的分枝杆菌物种的生物降解潜力，中和环境化学污染物。
Anthropogenic origin pollutants including pesticides, heavy metals, pharmaceuticals and industry chemicals impose many risks to human health and environment and bioremediation has been considered the strategy of choice to reduce the risk of hazardous chemicals. In the current study, we aimed to screen and characterize mycobacteria from the diverse range of Iranian aquatic and terrestrial ecosystems with harsh and unfavorable environmental conditions that can be utilized for biodegradation of target pollutants. Mycobacteria were isolated from a collection of 90 environmental samples and identified to the species level using conventional microbiological and molecular methods including the PCR amplification of hsp65 and sequence analysis of, 16S rRNA genetic markers. The growth rate of the isolates in presence of pollutants, chromatography, Gibbs and turbidometric methods were used to assess their biodegradation activity. A total of 39 mycobacterial isolates (43.3%) were recovered from 90 samples that belonged to 21 various species consisting of M. fortuitum; 6 isolates, M. flavescens and M. paragordonae; 4 isolates each, M. monacense, M. fredriksbergense and M. aurum; 2 isolates each, 7 single isolates of M. conceptionense, M. porcinum, M. simiae, M. celeriflavum, M. novocastrense, M. neoaurum, M. obuense and 12 isolates that belonged to 8 unknown potentially novel mycobacterial species. The isolates were categorized in three groups based on their bioremediation activity, i.e., 5 (12.8%) organisms without biodegradation activity, 20 (51.2%) organisms with previously reported biodegradation activity, and 14 (35.9%) organisms that showed biodegradation activity but not previously reported. Our results showed that the Iranian ecosystems harbor a good reservoir of diverse mycobacterial species with biodegrading potentiality for neutralizing environmental chemical pollutants.
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影响因子：
2.6
作者：
Chen, Liwei;Cai, Tianming;Wang, Qingling
通讯作者：
Wang, Qingling
影响因子：
9.4
作者：
Khan, IUH;Yadav, JS
通讯作者：
Yadav, JS
影响因子：
9.4
作者：
Adékambi, T;Stein, A;Drancourt, M
通讯作者：
Drancourt, M
DOI：
10.1099/00221287-28-2-333
发表时间：
1962-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
BOJALIL, LF;CERBON, J;TRUJILLO, A
通讯作者：
TRUJILLO, A
影响因子：
4.4
作者：
HARTMANS, S;DEBONT, JAM
通讯作者：
DEBONT, JAM