Deciphering transcriptional regulators of banana fruit ripening by regulatory network analysis.
Deciphering transcriptional regulators of banana fruit ripening by regulatory network analysis.
复制标题
通过调节网络分析的香蕉水果成熟的解密转录调节剂。
DOI：
10.1111/pbi.13477
复制
发表时间：
2021-03
影响因子：
13.8
通讯作者：
Lu WJ
中科院分区：
文献类型：
--
作者：
Kuang JF;Wu CJ;Guo YF;Walther D;Shan W;Chen JY;Chen L;Lu WJ
水果成熟是水果生产和销售的关键阶段。研究表明，果实成熟是一个高度协调的过程，主要在转录水平上受到调控，转录因子在其中起着重要作用。因此，确定关键的转录因子调节果实成熟，以及它们相关的调控网络，承诺有助于更好地了解果实成熟。在这项研究中，时间基因表达分析进行调查香蕉果实成熟的目的是辨别的全球架构的基因调控网络的果实成熟。八个时间点进行了分析，涵盖了表型的动态变化，相关的生理和已知的成熟标记基因的水平。结合加权基因共表达网络分析（WGCNA）和顺式基序分析的结果，并结合EMSA、Y1H、烟草、香蕉反式激活实验结果，构建了香蕉果实成熟调控网络，从中筛选出25个转录因子，通过调节不同的成熟相关通路来调控成熟过程。我们的研究首次提出了香蕉果实成熟过程中基因调控网络的全球观点，这可能为有针对性地操纵果实成熟以实现更高的香蕉和减少损失的香蕉商业化提供基础。
Fruit ripening is a critical phase in the production and marketing of fruits. Previous studies have indicated that fruit ripening is a highly coordinated process, mainly regulated at the transcriptional level, in which transcription factors play essential roles. Thus, identifying key transcription factors regulating fruit ripening as well as their associated regulatory networks promises to contribute to a better understanding of fruit ripening. In this study, temporal gene expression analyses were performed to investigate banana fruit ripening with the aim to discern the global architecture of gene regulatory networks underlying fruit ripening. Eight time points were profiled covering dynamic changes of phenotypes, the associated physiology and levels of known ripening marker genes. Combining results from a weighted gene co‐expression network analysis (WGCNA) as well as cis‐motif analysis and supported by EMSA, Y1H, tobacco‐, banana‐transactivation experimental results, the regulatory network of banana fruit ripening was constructed, from which 25 transcription factors were identified as prime candidates to regulate the ripening process by modulating different ripening‐related pathways. Our study presents the first global view of the gene regulatory network involved in banana fruit ripening, which may provide the basis for a targeted manipulation of fruit ripening to attain higher banana and loss‐reduced banana commercialization.
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DOI：
10.1073/pnas.1316278111
发表时间：
2014-02-11
影响因子：
11.1
作者：
Franco-Zorrilla, Jose M.;Lopez-Vidriero, Irene;Solano, Roberto
通讯作者：
Solano, Roberto
影响因子：
9.4
作者：
Jourda, Cyril;Cardi, Celine;Yahiaoui, Nabila
通讯作者：
Yahiaoui, Nabila
影响因子：
7.4
作者：
Li, Qiang;Byrns, Brook;Fowler, D. Brian
通讯作者：
Fowler, D. Brian
影响因子：
14.9
作者：
Jin J;Zhang H;Kong L;Gao G;Luo J
通讯作者：
Luo J
影响因子：
64.8
作者：
D'Hont, Angelique;Denoeud, France;Wincker, Patrick
通讯作者：
Wincker, Patrick