In Vivo Cell Fate Tracing Provides No Evidence for Mesenchymal to Epithelial Transition in Adult Fallopian Tube and Uterus.
In Vivo Cell Fate Tracing Provides No Evidence for Mesenchymal to Epithelial Transition in Adult Fallopian Tube and Uterus.
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DOI：
10.1016/j.celrep.2020.107631
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发表时间：
2020-05-12
期刊：
影响因子：
8.8
通讯作者：
Tanwar PS
中科院分区：
文献类型：
--
作者：
Ghosh A;Syed SM;Kumar M;Carpenter TJ;Teixeira JM;Houairia N;Negi S;Tanwar PS
间充质-上皮转化(MET)被认为与输卵管(输卵管)和子宫上皮的维持、修复和癌变有关。然而，缺乏确凿的证据证明这些器官中的间充质细胞转化为上皮细胞。利用由女性生殖道间充质细胞标志基因(AMHR2、CSPG4和PDGFRβ)驱动的报告基因进行胚胎细胞谱系追踪，我们发现这些报告基因在出生时也在一些输卵管和子宫上皮细胞中表达。这些间充质报告阳性的上皮细胞在多胎妊娠的成年小鼠中保持不变，对卵巢激素产生反应，并形成类器官。然而，在成年小鼠中诱导间充质细胞标记时，在任何输卵管或子宫上皮中都没有标记的上皮细胞。从成年标记的小鼠身上生长出来的有机物质对间充质报告也是阴性的。总的来说，我们的工作没有在成人输卵管和子宫上皮中发现MET的确凿证据。间充质-上皮转化(MET)被认为参与了女性生殖器官上皮的维持和再生。在这里，戈什等人。通过体内细胞谱系追踪和器官类物质的研究，未发现在成人输卵管和子宫上皮中存在MET的确凿证据。
The mesenchymal to epithelial transition (MET) is thought to be involved in the maintenance, repair, and carcinogenesis of the fallopian tube (oviduct) and uterine epithelium. However, conclusive evidence for the conversion of mesenchymal cells to epithelial cells in these organs is lacking. Using embryonal cell lineage tracing with reporters driven by mesenchymal cell marker genes of the female reproductive tract (AMHR2, CSPG4, and PDGFRβ), we show that these reporters are also expressed by some oviductal and uterine epithelial cells at birth. These mesenchymal reporter-positive epithelial cells are maintained in adult mice across multiple pregnancies, respond to ovarian hormones, and form organoids. However, no labeled epithelial cells are present in any oviductal or uterine epithelia when mesenchymal cell labeling was induced in adult mice. Organoids developed from mice labeled in adulthood were also negative for mesenchymal reporters. Collectively, our work found no definitive evidence of MET in the adult fallopian tube and uterine epithelium. Mesenchymal to epithelial transition (MET) is postulated to be involved in the maintenance and regeneration of the epithelium of female reproductive organs. Here, Ghosh et al. report no definitive evidence of MET in the adult epithelium of oviduct and uterus using in vivo cell lineage tracing and organoids.
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影响因子：
--
作者：
Hua Y;Choi PW;Trachtenberg AJ;Ng AC;Kuo WP;Ng SK;Dinulescu DM;Matzuk MM;Berkowitz RS;Ng SW
通讯作者：
Ng SW
影响因子：
2.7
作者：
Guioli, Silvana;Sekido, Ryohei;Lovell-Badge, Robin
通讯作者：
Lovell-Badge, Robin
影响因子：
64.5
作者：
Kretzschmar, Kai;Watt, Fiona M.
通讯作者：
Watt, Fiona M.
DOI：
10.1097/01.pgp.0000101082.35393.84
发表时间：
2004-01-01
影响因子：
2.4
作者：
Carcangiu, ML;Radice, P;Pasini, B
通讯作者：
Pasini, B
影响因子：
2.7
作者：
Orvis, Grant D.;Behringer, Richard R.
通讯作者：
Behringer, Richard R.