Ultrasound promotes germination of aging Pinus tabuliformis seeds is associated with altered lipid metabolism.
Ultrasound promotes germination of aging Pinus tabuliformis seeds is associated with altered lipid metabolism.
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超声会促进衰老的松果状蛋白酶种子的发芽与脂质代谢改变有关。
DOI：
10.1016/j.ultsonch.2023.106310
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发表时间：
2023-03
影响因子：
8.4
通讯作者：
Liu, Zhaojun
中科院分区：
文献类型：
--
作者：
Zhang, Huahai;Mo, Weiyi;Liao, Shaoming;Jia, Zhongtao;Zhang, Wenjie;Zhang, Shuoxin;Liu, Zhaojun
一定强度的超声波处理可使自然老化7年的油松种子萌发率提高约3倍，转录组和脂质组分析表明油松种子萌发过程与脂质代谢的改变有关，超声波可引起种子萌发过程中脂质组的重编程，超声波处理可使油松种子萌发过程中脂质组的重编程发生改变。种子萌发是高等植物建立新生物体必不可少的生物学过程，尤其是对保存在基因库中多年的老化种子而言，其意义更为重大。本文研究了超声波处理对华北地区大规模飞播造林中油松老化种子萌发的诱导作用。结果表明，一定强度的超声波可以使老化种子的发芽率提高3倍左右。有趣的是，尽管我们的转录组和脂质组分析显示，通过偏最小二乘判别分析（PLSDA）分析，可以在发芽后1天观察到对照和超声处理之间的差异，但大多数（75%或69%）显著改变的基因或脂质通常在它们之间共享。进一步分析两种处理之间的共同改变的脂质显示超声波引起的脂质在萌发过程中的变化。我们的研究还揭示了大量的超声波相关基因和脂质，可能参与发芽促进过程。我们假设超声波激发种子脂质体，进一步提高种子萌发的油松。我们的研究为超声波诱导脂质体变化在种子萌发中的作用提供了新的内容。此外，我们还提供了一种新的方法来提高老化种子的发芽率，这可能有利于种子库的保存。
Certain strength of ultrasound enhanced the germination of 7 years’ naturally aged Pinus tabuliformis seeds for about 3 times; The germination process of Pinus tabuliformis seeds is associated with altered lipid metabolism according to transcriptome and lipidome analysis; Ultrasound provokes lipidome reprogramming during seed germination; Seed germination is an essential biological process for establishing new organisms of higher plants, it is especially significant for those aged seeds stored in gene banks for years. In this study, we investigated ultrasound treatment induced germination for aged Pinus tabuliformis seeds, which has been used in large scale aircraft sowing based afforestation in North China over 30 years’ ago without knowing possible mechanisms. We showed certain strength of ultrasound could increase the germination rate of aged seeds for about 3 times compare with control. Interestingly, although our transcriptome and lipidome analysis showed the differences between control and ultrasound treatments can be observed 1 day after germination by partial least squares discriminant analysis (PLSDA) analysis, majority (75 % or 69 %) of the significantly altered genes or lipids were commonly shared between them. Further analysis for the commonly altered lipids between both treatments showed ultrasound provoked the variations of lipids during germination process. Our investigation also revealed large amount of ultrasound-related genes and lipids that might be involved in germination promotion process. We hypothesis ultrasound provokes seed lipidome which further increases seed germination of Pinus tabuliformis. Our study provides new insides into the role of ultrasound induced lipidome change in seed germination. Moreover, we provide a new method to improve germination of aged seeds which might benefit preservation of seeds in gene banks.
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