Malondialdehyde in exhaled breath condensate and urine as a biomarker of air pollution induced oxidative stress.
Malondialdehyde in exhaled breath condensate and urine as a biomarker of air pollution induced oxidative stress.
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呼出气冷凝物和尿液中的丙二醛作为空气污染引起的氧化应激的生物标志物
DOI：
10.1038/jes.2012.127
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发表时间：
2013-05
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
空气污染物对人类健康产生不利影响的潜在机制仍知之甚少。氧化应激被认为是一种潜在机制，它可能通过活性氧物质促进脂质过氧化，导致丙二醛（MDA）的形成，而丙二醛会在生物体液（如尿液和呼出气冷凝液（EBC））中排出。进行了一项队列研究，以检验EBC和尿液中MDA的浓度是否分别与北京奥运会空气污染控制措施带来的空气污染水平变化相关。在奥运会前、奥运会期间和奥运会后三个时期，分别对125名健康成年人采集两次EBC和尿液样本。使用线性混合效应模型估计了MDA的特定时期均值以及与24小时平均污染物浓度增加相关的MDA水平变化。从奥运会前到奥运会期间，当大多数污染物浓度降低时，EBC中的MDA和尿液中的MDA分别显著降低了24%（P < 0.0001）和28%（P = 0.0002）。从奥运会期间到奥运会后，当大多数污染物浓度增加时，EBC中的MDA和尿液中的MDA分别增加了28%（P = 0.094）和55%（P = 0.046）。此外，除臭氧外，所有污染物浓度每增加一个四分位距（IQR），EBC中MDA的最大增加幅度从10%（95%置信区间：2%，18%）到19%（95%置信区间：14%，25%）不等。尿液中MDA与污染物浓度IQR增加相关的最大增加幅度从9%（95%：0.3%，19%）到15%（95%置信区间：3%，28%）不等。这些发现支持了EBC中的MDA作为呼吸道氧化应激生物标志物以及尿液中的MDA作为与健康年轻人空气污染暴露相关的全身性氧化应激生物标志物的有效性。在普通人群中，EBC和尿液样本都可以无创采集。
Underlying mechanisms by which air pollutants adversely affect human health remain poorly understood. Oxidative stress has been considered as a potential mechanism that may promote lipid peroxidation by reactive oxygen species, leading to the formation of malondialdehyde (MDA) that is excreted in biofluids (eg, urine and exhaled breath condensate (EBC)). A panel study was conducted to examine whether concentrations of MDA in EBC and urine were associated, respectively, with changes in air pollution levels brought by the Beijing Olympic air pollution control measures. EBC and urine samples from 125 healthy adults were collected twice in each of the pre-, during-, and post-Olympic periods. Period-specific means of MDA and changes in MDA levels associated with increases in 24-h average pollutant concentrations were estimated using linear mixed-effects models. From the pre-to the during-Olympic period, when concentrations of most pollutants decreased, EBC MDA and urinary MDA significantly decreased by 24%(P< 0.0001) and 28%(P= 0.0002), respectively. From the during-Olympic to the post-Olympic period, when concentrations of most pollutants increased, EBC MDA and urinary MDA increased by 28%(P= 0.094) and 55%(P= 0.046), respectively. Furthermore, the largest increases in EBC MDA associated with one interquartile range (IQR) increases in all pollutants but ozone ranged from 10%(95% CI: 2%, 18%) to 19%(95% CI: 14%, 25%). The largest increases in urinary MDA associated with IQR increases in pollutant concentration ranged from 9%(95%: 0.3%, 19%) to 15%(95% CI: 3%, 28%). These findings support the utility of EBC MDA as a biomarker of oxidative stress in the respiratory tract and urinary MDA as a biomarker of systemic oxidative stress in relation to air pollution exposure in healthy young adults. Both EBC and urine samples can be collected noninvasively in the general population.
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影响因子：
10.4
作者：
Chen C;Arjomandi M;Balmes J;Tager I;Holland N
通讯作者：
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影响因子：
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作者：
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DOI：
10.1164/rccm.200611-1627oc
发表时间：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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